Hippocampal Gene expression analysis related with redox regulation in excitatory synaptic circuit of epileptogenesis

癫痫发生兴奋性突触回路氧化还原调节相关海马基因表达分析

• 批准号: 20591372
• 负责人: UEDA Yuto
• 金额: $ 2.91万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591372/
• 关键词: てんかん; グルタミン酸; GABA; トランスポーター; レドックス制御; チオレドキシン; レドックス抑制

As well known the knockdown EAAC-1 by anti-sense induces epileptic convulsion in rats, EAAC-1 is important protein to connect glutamate re-uptake with GABA synthesis. It is important study to elucidate the role of glutamate transporter associated protein 3-18 (GTRAP3-18), because GTRAP3-18 inhibitory regulates the glutamate re-uptake through EAAC-1 into GABAergic neurons. Hippocampal glutamate and GABA basal release in GTRAP3-18 knockdown group higher rather than those of sense-injected group suggested knockdown of GTRAP3-18 promotes GABA synthesis. Sustenance of high seizure susceptibility in the PTZ kindled state and the existence of inter-paroxysmal period could be explained by synchronization of glutamatergic neuron caused by the enhanced GABAergic system.

众所周知，反义敲除EAAC-1会引起大鼠癫痫性惊厥，EAAC-1是连接谷氨酸再摄取和GABA合成的重要蛋白质。阐明谷氨酸转运蛋白相关蛋白 3-18 (GTRAP3-18) 的作用是一项重要的研究，因为 GTRAP3-18 抑制性调节通过 EAAC-1 进入 GABA 神经元的谷氨酸再摄取。 GTRAP3-18 敲低组的海马谷氨酸和 GABA 基础释放高于正义注射组，表明 GTRAP3-18 敲低促进 GABA 合成。 PTZ 点燃状态下高癫痫易感性的维持和阵发间期的存在可以通过增强的 GABA 能系统引起的谷氨酸能神经元同步来解释。

项目成果

Redox regulation and anti-convulsive effect of zonisamide.

唑尼沙胺的氧化还原调节和抗惊厥作用。

• 发表时间: 2007
• 期刊: Redox and ZNS 1
• 作者: Ueda;Y.
• 通讯作者: Y.

Affect of dissolved oxygen on the reduction of nitroxide by vitamin C : In vitro model for acute ischamia.

溶解氧对维生素 C 还原硝基氧的影响：急性缺血的体外模型。

• 发表时间: 2010
• 作者: Nakajima;A.;Masuda;Y.;Matsuda;E.;Ueda;Y.;Sameshima;H.;Ikenoue;T.
• 通讯作者: T.

Hydrogen-Related Enhancement of In Vivo Antioxidant Ability in the Brain of Rats Fed Coral Calcium Hydride

• DOI: 10.1007/s11064-010-0204-5
• 发表时间: 2010-07
• 期刊: Neurochemical Research
• 影响因子: 4.4
• 作者: Y. Ueda;A. Nakajima;T. Oikawa

Differential molecular regulation of glutamate in kindling resistant rats

• DOI: 10.1016/j.brainres.2010.11.085
• 发表时间: 2011-02-23
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Doi, Taku;Ueda, Yuto;Willmore, L. James
• 通讯作者: Willmore, L. James

Levetiracetam enhances endogenoantioxidant in the hippocampusrats : in vivo evaluation by brain microdialysis combined with ESR spectroscopy.

左乙拉西坦增强海马大鼠的内源性抗氧化剂：通过脑微透析结合 ESR 光谱进行体内评估。

• 发表时间: 2009
• 期刊: Brain Res 1266
• 作者: Ueda;Y.;Doi;T.;Takaki;M.;Nagatomo;K.;Nakajima;A.;Willmore;L.J.
• 通讯作者: L.J.