Isolation and characterization of murine hepatic stem cells (HPCs) based on a new surface antigen.

基于新表面抗原的鼠肝干细胞 (HPC) 的分离和表征。

• 批准号: 20591610
• 负责人: YASUCHIKA Kentaro
• 金额: $ 3万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591610/
• 关键词: 肝幹細胞; 表面抗原; gp38

Previously, we clarified the surface antigen profiles of hepatic progenitor cells (HPCs) in fetal liver tissue as the CD49f+/CD45-/Thyl- cell fraction. In this study, we represented that more immature HPCs could be detected by using a novel surface antigen gp38 which was expressed in fetal livers but not in adult livers. Furthermore, the characterization of gp38-positive HPCs revealed that activation of Wnt signal pathway was related to the maintenance the undifferentiated state of HPCs.

之前，我们将胎儿肝组织中肝祖细胞 (HPC) 的表面抗原谱阐明为 CD49f+/CD45-/Thyl- 细胞组分。在这项研究中，我们表明使用一种新型表面抗原 gp38 可以检测到更多未成熟的 HPC，该抗原在胎儿肝脏中表达，但在成人肝脏中不表达。此外，gp38阳性HPCs的表征表明Wnt信号通路的激活与HPCs未分化状态的维持有关。

项目成果

A transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers.

• DOI: 10.1007/s11626-010-9354-7
• 发表时间: 2011-01
• 期刊: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
• 影响因子: 2.1
• 作者: Konishi, Sayuri;Yasuchika, Kentaro;Ishii, Takamichi;Fukumitsu, Ken;Kamo, Naoko;Fujita, Naoya;Ikai, Iwao;Uemoto, Shinji
• 通讯作者: Uemoto, Shinji

In vitro hepatic maturation of human embryonic stem cells by using a mesenchymal cell line derived from murine fetal livers

• DOI: 10.1007/s00441-009-0906-7
• 发表时间: 2010-03-01
• 期刊: CELL AND TISSUE RESEARCH
• 影响因子: 3.6
• 作者: Ishii, Takamichi;Yasuchika, Kentaro;Nakatsuji, Norio
• 通讯作者: Nakatsuji, Norio

Isolation of hepatic stem cells using novel surface antigen : gp38

使用新型表面抗原：gp38 分离肝干细胞

• 发表时间: 2008
• 作者: Konishi S;et. al.
• 通讯作者: et. al.

Variation in hepatic differentiation propensity among human induced pluripotent stem cell (iPSC) lines

人诱导多能干细胞 (iPSC) 系肝分化倾向的变化

• 发表时间: 2010
• 作者: 梶原正俊;石井隆道;猪飼伊和夫;上本伸二;山中伸弥
• 通讯作者: 山中伸弥

Comparative study of transplantation of cells at various differentitation stages into mice with induced lethal liver damage

不同分化阶段细胞移植致致死性肝损伤小鼠的比较研究

• 发表时间: 2010
• 作者: 上村良;石井隆道;安近健太郎;猪飼伊和夫
• 通讯作者: 猪飼伊和夫