Early effects of the ex vivo evaluation system on graft function after swine lung transplantation

离体评价系统对猪肺移植术后移植物功能的早期影响

• 批准号: 20591671
• 负责人: OTO Takahiro
• 金额: $ 2.91万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591671/
• 关键词: 肺移植; 臓器保存; 機械灌流; ドナー; 体外循環墨; 心臓死ドナー; 肺機能評価; グラフト; 心停止下臓器提供; 心停止ドナー; 人工心肺; 体外循環; 肺機能; 肺保存; 心臓死

Objectives : Ex vivo lung evaluation (ex vivo) has been developed as a useful method by which to assess lungs from donation after cardiac death (DCD) donors prior to transplant. However, the safety of the ex vivo circulation itself on grafts has not been fully investigated. The aim of this study is to evaluate the effects of the ex vivo circuit using a swine lung transplant model.Methods : Lungs with or without 2-hour warm ischemia were used. To assess posttransplant graft function, the left lung was transplanted after 2-hour ex vivo or cold preservation; blood gas analysis of the left pulmonary vein (PO_2) was performed during the 6-hour posttransplant follow-up period. Data were compared between the ex vivo (+) and ex vivo (-) groups.Results : PO_2/FiO_2 in the ex vivo (-) group was significantly greater than that in the ex vivo (+) group until 3 hours after transplant. The PO_2/FiO_2 levels in both groups then increased and became similar at 6 hours after transplant regardless of whether ischemic or nonischemic lungs (p<0.001 and p=0.004, respectively) were used.Conclusions : Negative effects of the ex vivo system was limited and seen only immediate posttransplant period. Therefore, in DCD swine lung transplantation, the ex vivo system appears to be safe.

目的：离体肺评价（ex vivo）已被开发为一种有用的方法，通过该方法可以在移植前评估来自心源性死亡（DCD）供体的肺。然而，离体循环本身对移植物的安全性尚未得到充分研究。本研究的目的是评估离体电路使用猪肺移植model.Methods的效果：肺有或无2小时热缺血。为评估移植后移植物的功能，将左肺在离体或冷保存2小时后移植，在移植后6小时的随访期内进行左肺静脉血气分析（PO_2）。结果：离体（-）组的PO 2/FiO 2在移植后3 h显著高于离体（+）组;两组的PO_2/FiO_2水平在移植后6小时开始升高并变得相似（分别为p<0.001和p=0.004）。因此，在DCD猪肺移植中，离体系统似乎是安全的。

项目成果

Early effects of the ex vivo evaluation system on graft function after swine lung transplantation

离体评价系统对猪肺移植术后移植物功能的早期影响

• 期刊: European Journal of Cardio-thoracic Surgery (掲載確定)
• 作者: 大谷真二、大藤剛宏、柿下大一;ほか
• 通讯作者: ほか

Suppression of Inflammatory Cytokines During Ex Vivo Lung Perfusion With an Adsorbent Membrane

• DOI: 10.1016/j.athoracsur.2010.02.077
• 发表时间: 2010-06-01
• 期刊: ANNALS OF THORACIC SURGERY
• 影响因子: 4.6
• 作者: Kakishita, Tomokazu;Oto, Takahiro;Miyoshi, Shinichiro
• 通讯作者: Miyoshi, Shinichiro

Does the ex-vivo lung evaluation system affect post-transplant graft function in swine donation after cardiac death (DCD) lung transplantation?

离体肺评估系统是否会影响猪心脏死亡（DCD）肺移植后捐献的移植后移植功能？

• 发表时间: 2010
• 作者: Otani S;Oto T
• 通讯作者: Oto T

Effects of ex-vivo lung evaluation system on post-transplant graft function in swine donation after cardiac death (DCD) lung transplantation

离体肺评价系统对猪心脏死亡（DCD）肺移植捐献后移植物功能的影响

• 发表时间: 2010
• 作者: 大谷真二、大藤剛宏、三好新一郎;ほか
• 通讯作者: ほか

Ex-vivo肺灌流回路における灌流液中サイトカインの肺機能に与える影響

离体肺灌注回路中灌注液中细胞因子对肺功能的影响

• 发表时间: 2008
• 作者: 柿下大一;大藤剛宏
• 通讯作者: 大藤剛宏