Detection of immunoresponse to flame-shift proteins in MSI positive endometrial cancer patients.

为了检测MSI阳性子宫内膜癌患者对火焰移位蛋白的特异性免疫应答，我们合成了MSI相关的火焰移位肽，并在MSI阳性患者血清中检测了针对这些肽的抗体。但MSI阳性患者均未检测到MSI相关肽的特异性抗体。应用SEREX法检测MSI阳性子宫内膜癌移植瘤SCID小鼠血清中MYEOV蛋白。MYEOV与子宫内膜癌细胞的侵袭和迁移有关，是子宫内膜癌的预后因素。

To detect specific immune response to flame-shift proteins in MSI positive endometrial cancer patients, we synthesized MSI associated flame-shift peptides, and evaluated antibodies to these peptides in MSI positive patients' sera. But none of MSI positive patients had specific antibodies to MSI associated peptides. We detected MYEOV protein by SEREX method using SCID mouse sera ,these mice was transplantated MSI positive endometrial cancer tissues. MYEOV was associated with invasion and migration in endometrial cancer cells, and was prognostic factor in endometrial cancer.