Investigation of immune tolerance in the islet transplantation using adipose tissue derived stem cell.

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Cocultivation OP9-DL1 cell and human ADSC was performed, but pollution in culture enforced to take time. As an allied experiment, the cocultivation ADSC with the pancreatic islet, ADSC with hepatocytes, and the liver segmental resection+I/R+ ADSC, STZ injured pancreas+ADSC model were performed. Trophic effect and homing effect were investigated in remnant liver and injured pancreas.Cocultivation: cell viability was high in both groups, and VEGF density was high prices out of the culture fluid in both groups. When anti VEGF antibody was added in culture fluid, pancreatic islet viabilty decreased. Thus it was thought that VEGF signal might be key signal of the pancreatic islet protection. In the liver segmental resection+I/R+ADSC model, ADSC showed homing in remnant liver. The hepatocytes with SDF-1appearance changed into non-substance cell. There was no homing of ADSC in STZ injured pancreas + ADSC model.ADSC showed organ-specifically trophic, homing, and a cell protection effect.