Investigation of immune tolerance in the islet transplantation using adipose tissue derived stem cell.

使用脂肪组织来源的干细胞研究胰岛移植中的免疫耐受性。

• 批准号: 23592010
• 负责人: IWATA Takashi
• 金额: $ 3.24万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592010/
• 关键词: 膵島移植; 拒絶反応; 脂肪由来幹細胞; 免疫寛容; trophic効果; homing効果; OP9DL1細胞; VEGF signal; 脂肪由来幹細胞（ADSC）; 調節性T細胞 Treg; helper T細胞; suppressor T細胞

Cocultivation OP9-DL1 cell and human ADSC was performed, but pollution in culture enforced to take time. As an allied experiment, the cocultivation ADSC with the pancreatic islet, ADSC with hepatocytes, and the liver segmental resection+I/R+ ADSC, STZ injured pancreas+ADSC model were performed. Trophic effect and homing effect were investigated in remnant liver and injured pancreas.Cocultivation: cell viability was high in both groups, and VEGF density was high prices out of the culture fluid in both groups. When anti VEGF antibody was added in culture fluid, pancreatic islet viabilty decreased. Thus it was thought that VEGF signal might be key signal of the pancreatic islet protection. In the liver segmental resection+I/R+ADSC model, ADSC showed homing in remnant liver. The hepatocytes with SDF-1appearance changed into non-substance cell. There was no homing of ADSC in STZ injured pancreas + ADSC model.ADSC showed organ-specifically trophic, homing, and a cell protection effect.

进行了共培养OP9-DL1细胞和人ADSC，但在培养中污染需要花费时间。作为一个盟军实验，执行了与胰岛，肝细胞ADSC的共培养ADSC，以及肝分段切除+I/R+ADSC，STZ损伤胰腺+ADSC模型。在残留的肝脏和受伤的胰腺中研究了营养作用和归巢效应。共培养：两组的细胞活力都很高，而VEGF密度在两组中的培养液中的价格很高。当在培养液中添加抗VEGF抗体时，胰岛胰岛可维质减少。因此，人们认为VEGF信号可能是胰岛保护的关键信号。在肝分段切除+I/R+ADSC模型中，ADSC显示了残留肝脏中的归巢。带有SDF-1出现的肝细胞变为非固定细胞。在STZ损伤胰腺 + ADSC模型中，ADSC没有归巢。ADSC显示了器官特异性营养，归巢和细胞保护效果。

项目成果

Characteristics of internal hernia after gastrectomy with Roux-en-Y reconstruction for gastric cancer

• DOI: 10.1007/s00464-013-3384-7
• 发表时间: 2014-06-01
• 期刊: SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
• 影响因子: 3.1
• 作者: Yoshikawa, Kozo;Shimada, Mitsuo;Eto, Syohei
• 通讯作者: Eto, Syohei