Imaging analysis of the electrolyte transport and protein secretions in salivary ducts to reveal their regulatory mechanisms

唾液管电解质运输和蛋白质分泌的成像分析揭示其调节机制

• 批准号: 20592180
• 负责人: TANIMURA Akihiko
• 金额: $ 3.08万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592180/
• 关键词: 歯科薬理学; 唾液腺; 導管; カルシウム; 分泌; イメージング; 蛍光タンパク質; プリン受容体; イノシトール三リン酸; 蛍光蛋白質

Using multiphoton microscopy, the present study established that rat salivary ductal cells exhibit spontaneous oscillations in intracellular Ca^<2+> concentration([Ca^<2+>] i). Pharmacological examinations indicate that purinergic receptors play major roles in these spontaneous Ca2+oscillations. It was also found that spontaneous elevations of[Ca^<2+>] i were well correlated with spontaneous cell swelling of ductal cells. These results indicate the possibility that the Ca^<2+> oscillations are induced by the spontaneous ATP release in salivary ductal cells in the resting state and regulate electrolyte reabsorption.

利用多光子显微镜，本研究证实了大鼠唾液导管细胞在细胞内Ca^<2+>浓度（[Ca^<2+>] i）时表现出自发振荡。药理学检查表明嘌呤能受体在这些自发Ca2+振荡中起主要作用。[Ca^<2+>] i的自发升高与导管细胞的自发细胞肿胀密切相关。这些结果表明，Ca^<2+>振荡可能是由唾液导管细胞在静息状态下自发释放ATP引起的，并调节电解质的重吸收。

项目成果

細胞質発現型IP_3バイオセンサーの精製とそれを用いた耳下腺腺房細胞のIP_3濃度測定への応用

细胞质表达IP_3生物传感器的纯化及其在腮腺腺泡细胞IP_3浓度测量中的应用

• 发表时间: 2009
• 作者: 根津顕弘;他
• 通讯作者: 他

The Development of FRET-Based IP3 Biosensors and Their Use for Monitoring IP3 Dynamics during Ca2+ Oscillations and Ca2+ Waves in Non-Excitable Cells

基于 FRET 的 IP3 生物传感器的开发及其用于监测不可兴奋细胞中 Ca2 振荡和 Ca2 波期间 IP3 动态的用途

• 发表时间: 2011
• 作者: A. Tanimura

Roles of purinergic receptors on the spontaneous and agonist-induced Ca^<2+> responses in rat salivary ducts

嘌呤能受体对大鼠唾液管自发和激动剂诱导的 Ca^2 反应的作用

• 发表时间: 2011
• 作者: Nakanishi H;Wu Z.;Tanimura A.
• 通讯作者: Tanimura A.

Mechanism of calcium waves and oscillations in non-excitable cells

非兴奋细胞中钙波和振荡的机制

• 发表时间: 2009
• 期刊: International Journal of Oral-Medical Science
• 作者: A.Yano;S.Kikuchi;Y.Yamashita;Y.Sakamoto;Y.Nakagawa;Y.Yoshida;松尾拡;Tanimura A.
• 通讯作者: Tanimura A.

Visualization of Ins(1,4,5)P_3 dynamics in living cells : two distinct pathways for Ins(1,4,5)P_3generation following mechanical stimulation of HSY-EA1 cells.

活细胞中 Ins(1,4,5)P_3 动力学的可视化：HSY-EA1 细胞机械刺激后 Ins(1,4,5)P_3 生成的两种不同途径。

• 发表时间: 2010
• 期刊: Journal of Cell Science
• 影响因子: 4
• 作者: Nezu A.;et al.
• 通讯作者: et al.