Regulation mechanism of differentiation of dental epithelial cells induced by glycoshingolipids.

糖脂诱导牙上皮细胞分化的调控机制

• 批准号: 20592405
• 负责人: KAMASAKI Yoko
• 金额: $ 3.08万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592405/
• 关键词: 糖脂質GM3; 歯原性上皮細胞; エナメル質; 神経栄養因子NT-4; アメロブラスチン; TrkB; LacCer; MAPK; GSL生合成阻害剤; 抗GM3抗体M2590; D-PDMP; Trk-B; p-75; 細胞内シグナリング

A number of growth factors, including neurotrophic factor NT-4, and their receptors mediate reciprocal epithelial-mesenchymal interactions that regulate the differentiation of ameloblasts and odontoblasts in tooth development. Glycosphingolipids are involved in a variety of membrane-associated cell physiological functions. In this study, we identified strong expressions of GM3 and LacCer in dental epithelium, which give rise to differentiation into ameloblasts. We obtained the results from some experiments suggesting that GM3 and LacCer are essential for NT-4-mediated Ambn gene expression and contribute to dental epithelial cell differentiation into ameloblasts.

许多生长因子，包括神经营养因子NT-4，及其受体介导的相互上皮间质的相互作用，调节成釉细胞和成牙本质细胞在牙齿发育的分化。鞘糖脂参与多种膜相关细胞生理功能。在这项研究中，我们确定了GM 3和LacCer在牙上皮中的强表达，这引起了成釉细胞的分化。我们从一些实验中得到的结果表明，GM 3和LacCer是NT-4介导的Ambn基因表达所必需的，并有助于牙上皮细胞分化成成釉细胞。