Study for regulating mechanism of mesenchymal cells differentiation

间充质细胞分化调控机制研究

• 批准号: 23593033
• 负责人: KAMASAKI Yoko
• 金额: $ 3.41万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23593033/
• 关键词: 糖脂質; GM3; LacCer; 神経栄養因子NT-4; 歯原性間葉細胞; DSPP; 糖脂質GM3; 上皮ー間葉相互作用; TrkB-FL; d-PDMP; TrkB-T1; TrkB－FL; TrkB－T１

We investigated the interactions of glycosphingolipids (GSLs) and neurotrophin 4 (NT-4) in the mouse mesenchymal cells.We showed that exogenously added GM3 (monosialoganglioside) and LacCer (lactosylceramide) suppressed the proliferation of mouse mesenchymal cells by BrdU incorporation assay, and that dental mesenchymal cell proliferation activities were significantly disturbed when NT-4 was added to the culture medium with GM3. We revealed that NT-4 increased expression of DSPP in the mouse mesenchymal cells by RT-PCR and those expression levels were enhanced when NT-4 was added to the culture medium with GM3 or LacCer. These results suggested that NT-4 might cooperate with GSLs on cell surface and regulate the differentiation. we examined the expression patterns of TrkB (TrkB can exist as alternatively truncated TrkB splice variants) on mouse mesenchymal cells by immunohistochemistry, and revealed that full-length TrkB were expressed, while truncated TrkB were absent.

我们研究了鞘糖脂（glycosphingolipids，GSL）和神经营养因子4（neurotrophin 4，NT-4）在小鼠牙间充质细胞中的相互作用，结果表明，外源性GM 3（monosialoganglioside，单唾液酸神经节苷脂）和乳糖神经酰胺（lactosylceramide，乳糖神经酰胺）均能抑制小鼠牙间充质细胞的增殖，当NT-4加入到含有GM 3的培养液中时，其增殖活性明显受到干扰。RT-PCR结果显示，NT-4可促进小鼠骨髓间充质细胞DSPP的表达，当NT-4与GM 3或LacCer共同作用时，DSPP的表达水平明显升高。提示NT-4可能与细胞表面的GSL协同作用，调控细胞的分化。我们通过免疫组织化学检测了TrkB在小鼠间充质细胞上的表达模式（TrkB可以作为可选的截短的TrkB剪接变体存在），并且揭示了全长TrkB被表达，而截短的TrkB不存在。

项目成果

Pannexin 3 Inhibits Proliferation of Osteoprogenitor Cells by Regulating Wnt and p21 Signaling

• DOI: 10.1074/jbc.m113.523241
• 发表时间: 2014-01-31
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Ishikawa, Masaki;Iwamoto, Tsutomu;Yamada, Yoshihiko
• 通讯作者: Yamada, Yoshihiko

Differentiation of Induced Pluripotent Stem Cells Into Dental Mesenchymal Cells

• DOI: 10.1089/scd.2011.0210
• 发表时间: 2012-05-01
• 期刊: STEM CELLS AND DEVELOPMENT
• 影响因子: 4
• 作者: Otsu, Keishi;Kishigami, Ryota;Harada, Hidemitsu
• 通讯作者: Harada, Hidemitsu

Neurotrophic signaling is regulated by glycosphingolipids in dental mesenchymal l cells

神经营养信号由牙科间充质细胞中的鞘糖脂调节

• 发表时间: 2014
• 作者: 釜崎陽子;山田亜矢;西口美由季;藤原 卓;福本 敏
• 通讯作者: 福本 敏

Glycosphingolipids regulate ameloblastin expression in dental epithelial cells

鞘糖脂调节牙上皮细胞中的成釉细胞蛋白表达

• DOI: 10.1177/0022034511424408
• 发表时间: 2012
• 期刊: Journal of Dental Research
• 影响因子: 7.6
• 作者: Kamasaki;et al
• 通讯作者: et al

Cell dynamics in cervical loop epithelium during transition from crown to root: implications for Hertwig's epithelial root sheath formation

• DOI: 10.1111/jre.12003
• 发表时间: 2013-04-01
• 期刊: JOURNAL OF PERIODONTAL RESEARCH
• 影响因子: 3.5
• 作者: Sakano, M.;Otsu, K.;Harada, H.
• 通讯作者: Harada, H.