The chemical biology of low side effects compound in anti-fever drugs, which is derived from the analysis about fatal function by diclofenac.

退烧药中低副作用化合物的化学生物学，源于对双氯芬酸致命作用的分析。

• 批准号: 20611013
• 负责人: YANO Mihiro
• 金额: $ 2.83万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20611013/
• 关键词: インフルエンザウイルス; ジクロフェナクナトリウム; ウイルス放出障害; インフルエンザ脳症; 解熱剤; ジクロフェナック; ウイルス放出阻害; Caチャネルブロッカー; Naチャネルブロッカー; ライ症候群

To clarify the mechanisms of influenza encephalitis induced by diclofenac, we examined the effect of influenza virus and diclofenac on the disruption of tight junction (TJ) in human vascular endothelial cells in vitro. In this study, we found that influenza virus induced the degradation of TJ proteins, such as ZO1, leading to its disruption. On the other hands, diclofenac by itself inhibited the synthesis of TJ proteins, consequently accelerating the disruption of TJ induced by influenza infection in vascular endothelial cells.

为了阐明双氯芬酸诱导的流感脑炎的机制，我们检查了流感病毒和双氯芬酸对人血管内皮细胞中紧密连接（TJ）的影响。在这项研究中，我们发现流感病毒诱导了TJ蛋白（例如ZO1）的降解，导致其破坏。另一方面，双氯芬酸本身抑制了TJ蛋白的合成，因此加速了血管内皮细胞中流感感染引起的TJ的破坏。

项目成果

Boost of mucosal secretoty immunoglobulin A response by clarithromycin in paediatric influenza.

克拉霉素在小儿流感中增强粘膜分泌性免疫球蛋白 A 反应。

• 发表时间: 2009
• 期刊: Respirology 14(8)
• 作者: 柴崎忠雄;他;Takako Sawabuchi
• 通讯作者: Takako Sawabuchi

Influenza Virus-Cytokine-Protease Cycle in the Pathogenesis of Vascular Hyperpermeability in Severe Influenza

• DOI: 10.1086/656044
• 发表时间: 2010-10-01
• 期刊: JOURNAL OF INFECTIOUS DISEASES
• 影响因子: 6.4
• 作者: Wang, Siye;Le, Trong Quang;Kido, Hiroshi
• 通讯作者: Kido, Hiroshi

Cleavage of the SARS coronavirus spike glycoprotein by airway proteases enhances virus entry into human bronchial epithelial cells in vitro.

• DOI: 10.1371/journal.pone.0007870
• 发表时间: 2009-11-17
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Kam YW;Okumura Y;Kido H;Ng LF;Bruzzone R;Altmeyer R
• 通讯作者: Altmeyer R

Ibuprofen Increases Cisplatin-induced Apoptosis in Lung Adenocarcinoma by Suppressing Hsp70.

布洛芬通过抑制 Hsp70 增加顺铂诱导的肺腺癌细胞凋亡。

• 发表时间: 2008
• 作者: M Yano;H Endo;H Kido
• 通讯作者: H Kido

Novel type II transmembrane serine protease, MSPL and TMPRSS13, proteolytically activate membrane fusion activation of hemagglutinin of highly pathogenic avian influenza viruses and induce their multicycle replication.

新型 II 型跨膜丝氨酸蛋白酶 MSPL 和 TMPRSS13 通过蛋白水解激活高致病性禽流感病毒血凝素的膜融合激活并诱导其多周期复制。

• 发表时间: 2010
• 期刊: The Journal Virology
• 作者: Kikuchi K;Natsuga K;Shinkuma S;Nishie W;Kajita S;Sato H;Shimizu H;Yuushi Okumura
• 通讯作者: Yuushi Okumura