Development of the photochemical panning adapted to low affinity ligands and analysis of neural carbohydrates' functions

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We established the synthetic methods for photo-reactive carbohydrates, in which carbohydrate reducing end was cyclic form, i.e. natural form. Experiments using the new photo-reactive lactose ligand revealed that cyclic form of lactose showed high affinity and high specificity comparing acyclic form of lactose. We also analyzed the interaction between carbohydrates and Alzheimer's beta-amyloid peptides on the lipid membrane by atomic force microscope, and revealed that the interaction of ganglioside and amyloid peptide generated the specific aggregation on lipid layer.