Identification of protein constituting inclusions from formalin-fixed paraffin-embedded fronto-temporal dementia brain tissue using laser-capture microdissection

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Basophilic Inclusions (BIs) in front-temporal dementia with BIs were separated and collected by laser capture micro-dissection from formalin-fixed paraffin-embedded brain tissue. The identified proteins by liquid chromatography coupled to mass spectrometry and concerning proteins were examined immunohistochemically. The BIs were distinctly labeled with the antibodies against microtubule-associated protein and autophagy-related gene proteins. Our results imply that autophagy has been disrupted before the completion of autophagosome formation, which might contribute to BI formation in this disorder.