Analysis on the molecular mechanisms of wider dynamic range and higher time-resolution in cone photoreceptor cells.

锥体感光细胞更宽动态范围和更高时间分辨率的分子机制分析。

• 批准号: 20700350
• 负责人: TACHIBANAKI Shuji
• 金额: $ 2.75万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20700350/
• 关键词: 視細胞; 錐体; GRK; Sモジュリン; 順応; RGS9; cGMP; Sモミュリン・s26; 発色団代謝経路; ALOL反応

In vertebrate retina, there are two types of photoreceptor cells, called rods and cones. The time resolution of cone mediated vision is higher than that of rod-mediated vision. This higher time-resolution depends on shorter responses of cones. It is also known that the range of light-intensity that cone can mediate is wider than that of rod. In this study, the underlying mechanisms of cone-specific responses are studied. The results show that the lifetime of activated enzymes, working in the phototransduction mechanism, are shorter in cones than in rods. And It is also revealed that the enzymatic activities in the phototransduction mechanism are more highly modulated in cones than in rods. These findings show molecular bases of the cone specific photoresponses.

在脊椎动物视网膜中，有两种类型的感光细胞，称为视杆细胞和视锥细胞。视锥细胞介导视觉的时间分辨率高于视杆细胞介导视觉。这种较高的时间分辨率取决于视锥细胞的较短响应。我们还知道，视锥细胞所能传递的光强范围比视杆细胞所能传递的光强范围更广。在这项研究中，锥特异性反应的潜在机制进行了研究。结果表明，在光传导机制中起作用的活性酶在视锥细胞中的寿命比在视杆细胞中短。光传导机制中的酶活性在视锥细胞中受到的调节比在视杆细胞中受到的调节更大。这些发现显示了视锥细胞特异性光反应的分子基础。

项目成果

Highly efficient retinal metabolism in cones

• DOI: 10.1073/pnas.0806593105
• 发表时间: 2008-10-14
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Miyazono, Sadaharu;Shimauchi-Matsukawa, Yoshie;Kawamura, Satoru
• 通讯作者: Kawamura, Satoru

2種類の視物質キナーゼ(GRK1,GRK7)の活性の違いをもたらす領域の特定

鉴定导致两种视觉物质激酶（GRK1、GRK7）活性差异的区域

• 发表时间: 2009
• 作者: 瀬野亜希;竹本訓彦;橘木修志(発表者);河村悟;橘木修志
• 通讯作者: 橘木修志

Amino Acid Residues in GRK1/GRK7 Responsible for Interaction with S-modulin/Recoverin

GRK1/GRK7 中负责与 S-调节蛋白/恢复蛋白相互作用的氨基酸残基

• 期刊: Photochemistry and Photobiology (in press)
• 作者: Torisawa;A.;Arinobu;D;Tachibanaki;S.;Kawamura;S
• 通讯作者: S

High cGMP synthetic activity in carp cones

• DOI: 10.1073/pnas.0812781106
• 发表时间: 2009-07-14
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Takemoto, Norihiko;Tachibanaki, Shuji;Kawamura, Satoru
• 通讯作者: Kawamura, Satoru

Rod and cone photoreceptors : molecular basis of the difference in their さphysiology

视杆细胞和视锥细胞光感受器：生理学差异的分子基础

• 发表时间: 2008
• 期刊: Comparative Biochemistry and Physiology, Part A 150(4)
• 作者: Kawamura;S. and Tachibanaki;S.
• 通讯作者: S.