Study on sperm preservation at room temperature in the rodents

啮齿动物精子室温保存研究

• 批准号: 20700367
• 负责人: KANEKO Takehito
• 金额: $ 2.66万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20700367/
• 关键词: マウス; ラット; 精子; 保存; ICSI; フリーズドライ

Sperm preservation at room temperature is a powerful tool for bio-resource projects. Freeze-drying is a method which can preserve and transport sperm at room temperature without using liquid nitrogen. We studied preservation of sperm at room temperature by using freeze-drying. The fertility of freeze-dried sperm was maintained after preservation for 1 year at 4 oC. Offspring was obtained from oocytes fertilized with these sperm. Moreover, this study was described that normality of sperm chromatin is an important factor to preserve sperm at room temperature.

精子室温保存是生物资源项目的有力工具。冷冻干燥是一种在室温下不使用液氮保存和运输精子的方法。我们研究了冷冻干燥法在室温下保存精子。冷冻干燥精子在4 ℃保存1年后，其生育力仍保持不变。从与这些精子受精的卵母细胞中获得后代。此外，本研究还表明精子染色质的正常性是影响精子常温保存的重要因素。

项目成果

ラットフリーズドライ精子の長期保存後の受精能とICSI胚の培養条件の検討

长期保存大鼠冻干精子受精潜力及ICSI胚胎培养条件的检测

• 发表时间: 2008
• 作者: Takehito Kaneko;Shinya Kimura;Naomi Nakagata;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko;金子武人;金子武人;金子武人;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko;金子武人
• 通讯作者: 金子武人

The development of oocytes injected with freeze-dried rat sperm

注射冻干大鼠精子的卵母细胞的发育

• 发表时间: 2009
• 作者: Takehito Kaneko;Shinya Kimura;Naomi Nakagata;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko
• 通讯作者: Takehito Kaneko

Tolerance to freezing and maintenance of fertilizing ability in rat sperm

大鼠精子的冷冻耐受性和受精能力的维持

• 发表时间: 2009
• 作者: Takehito Kaneko;Shinya Kimura;Naomi Nakagata;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko
• 通讯作者: Takehito Kaneko

Resource banking and fertilization technuiques

资源库和施肥技术

• 发表时间: 2009
• 作者: Takehito Kaneko;Shinya Kimura;Naomi Nakagata;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko
• 通讯作者: Takehito Kaneko

The development of oocytes injected with freeze-dried rat sperm.

注射冻干大鼠精子的卵母细胞的发育。

• 发表时间: 2009
• 作者: Takehito Kaneko;Shinya Kimura;Naomi Nakagata;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko;金子武人;金子武人;金子武人;Takehito Kaneko;Takehito Kaneko;Takehito Kaneko
• 通讯作者: Takehito Kaneko