Effects of meiosis-activating sterol on rRNA gene transcription and the developmental potential of preimplantation porcine embryos

减数分裂激活甾醇对 rRNA 基因转录和植入前猪胚胎发育潜力的影响

• 批准号: 5377124
• 负责人: Professor Dr. Heiner Niemann
• 金额: --
• 依托单位: Institut für Nutztiergenetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2002
• 资助国家: 德国
• 起止时间: 2001-12-31 至 2004-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5377124?language=en
• 关键词: Effects; meiosis; activating; sterol; rRNA

Current in vitro production systems have deteriorating effects on the normality of the resulting embryos. Deviations from the normal activation of the genes encoding the Topoisomerase I, Polymerase I, Upstream binding factor, Fibrilarin, Nucleolin and Nucleophosmin may be involved in this effect. Employing immunocytochemistry and RT-PCR technology we will study the molecular mechanisms involved in re-initiation of the embryonic rRNA transcription in vivo and thus develop suitable markers for evaluation of the normality of the embryos produced under various in vitro conditions. Special emphasis will be devoted to the role of meiosis activating sterol (MAS) during early development of the porcine embryo in vitro. Modification of oocyte culture conditions will focus on improvement of the in vitro pig embryo production system to such a level that the embryos of a sufficient viability can be produced on a routine basis. These data will contribute to understanding of the cell and molecular mechanisms regulating porcine pre-implantation embryonic development, increase the number of markers suitable for evaluation of the normality of the embryos, improve the culture conditions and finally increase the normality of embryos used in biomedical research.

目前的体外生产系统对所产生的胚胎的正常性有恶化的影响。编码拓扑异构酶I、聚合酶I、上游结合因子、原纤蛋白、核仁素和核磷蛋白的基因的正常激活的偏离可能参与这种效应。采用免疫细胞化学和RT-PCR技术，我们将研究在体内重新启动的胚胎rRNA转录的分子机制，从而开发合适的标记物，用于评估在各种体外条件下产生的胚胎的正常性。特别强调的是减数分裂激活甾醇（MAS）在猪胚胎体外早期发育过程中的作用。卵母细胞培养条件的改进将集中于体外猪胚胎生产系统的改进，使其达到常规生产具有足够活力的胚胎的水平。这些数据将有助于了解调控猪植入前胚胎发育的细胞和分子机制，增加适合于评估胚胎正常性的标记物的数量，改善培养条件，最终提高用于生物医学研究的胚胎的正常性。