Identification of protein dynamics for enzymatic reaction using four-dimensional visualizing analysis

本课题旨在建立酶促反应机理中蛋白质动力学的可视化方法。在这种情况下，我们选择了含铜亚硝酸盐还原酶晶体作为测试案例，并收集了高亮度x射线诱导还原过程中的衍射数据。结果，我们成功地在不同的傅里叶电子密度图中观察到铜结合位点的一些明确的结构变化。

The objective for this research project is to establish the methods for visualizing the protein dynamics on enzymatic reaction mechanism. In this case, we selected a crystal for copper-containing nitrite reductase as a test case, and collected the diffraction data during high-brilliant x-ray induced reduction. As a result, we have succeeded in observation of some unambiguous structural changes at the copper binding sites in the difference fourier electron density maps.