Study of the role of two AR subtypes on spiggin synthesis in stickleback kidney

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Full length of ARαand ARβcDNAs were isolated from mature male stickleback kidney. It was showed by reporter gene assay that both were functional AR, and had almost same potency of transcriptional activation. The changes in the mRNA levels of spiggin and both ARs of immature female stickleback treated with androgen were measured and detected by quantitative RT-PCR and in situ hybridization. Spiggin mRNA levels increased in androgen dose and exposure duration-dependent manner. In contrast, the mRNA levels of both ARs were significantly decreased by androgen exposure. In addition, the levels of ARαwere about 10 times higher than that of ARβduring androgen exposure. These results suggested ARαwas essential for the spiggin synthesis in stickleback kidney.