Study of the role of two AR subtypes on spiggin synthesis in stickleback kidney

两种AR亚型对刺鱼肾脏spiggin合成的作用研究

• 批准号: 21510069
• 负责人: NAGAE Masaki
• 金额: $ 3.08万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21510069/
• 关键词: バイオマーカー; 環境ホルモン; イトヨ; 男性ホルモン; スピギン; バオマーカー

Full length of ARαand ARβcDNAs were isolated from mature male stickleback kidney. It was showed by reporter gene assay that both were functional AR, and had almost same potency of transcriptional activation. The changes in the mRNA levels of spiggin and both ARs of immature female stickleback treated with androgen were measured and detected by quantitative RT-PCR and in situ hybridization. Spiggin mRNA levels increased in androgen dose and exposure duration-dependent manner. In contrast, the mRNA levels of both ARs were significantly decreased by androgen exposure. In addition, the levels of ARαwere about 10 times higher than that of ARβduring androgen exposure. These results suggested ARαwas essential for the spiggin synthesis in stickleback kidney.

从成熟雄棘鱼肾中分离到完整的ar α和ar β cdna。报告基因分析表明，两者都是功能性AR，并且具有几乎相同的转录激活效力。采用定量RT-PCR和原位杂交技术检测雄激素处理后未成熟雌棘鱼spiggin和两种ar mRNA水平的变化。Spiggin mRNA水平随雄激素剂量和暴露时间的增加而增加。相比之下，雄激素暴露显著降低了两种ar的mRNA水平。此外，在雄激素暴露期间，ar α水平比ar β高约10倍。这些结果表明，ar α在棘鱼肾脏的spigin合成中起重要作用。

项目成果

トビハゼを用いた干潟における環境ホルモン汚染の影響評価

利用弹涂鱼评价环境激素污染对滩涂的影响

• 发表时间: 2011
• 作者: 岡本育子;齋藤義紀;高島由利子;鎌田綾;伊賀砂央里;〓洵;花井亮;黒田智明;通元夫;鈴木絢子・長江真樹・征矢野清
• 通讯作者: 鈴木絢子・長江真樹・征矢野清

Bisphenol A modulates expression of gonadotropin subunit genes in the hermaphroditic fish, Kryptolebias marmoratus.

• DOI: 10.1016/j.cbpc.2010.07.003
• 发表时间: 2010-11
• 期刊: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
• 作者: J. Rhee;R. Kim;J. Seo;H. Kang;Chang-Beom Park;K. Soyano;Jehee Lee;Youngmi Lee;Jae-Seong Lee

The effects of 17beta-estradiol on various reproductive parameters in the hermap hrodite fish Kryptolebias marmoratus.

17β-雌二醇对赫罗迪特鱼 Kryptolebias marmoratus 各种繁殖参数的影响。

• 发表时间: 2010
• 期刊: Aquat.Toxicol.
• 作者: Park CB;Aoki J;Lee JS;Nagae M;Lee YD;Sakakura Y;Hagiwara A;Soyano K
• 通讯作者: Soyano K

Choriogenin and vitellogenin in red lip mullet(Chelon haematocheilus): Purification, characterization, and evaluation as potential biomarkers for detecting estrogenic activity

红唇鲻鱼（Chelon haematocheilus）中的绒毛蛋白和卵黄蛋白原：纯化、表征和评估作为检测雌激素活性的潜在生物标志物

• 发表时间: 2009
• 期刊: Comparative Biochemistry and Physiology Part C
• 作者: Hong L.;Fujita T.;Wada T.;Amano H.;Hiramatsu N.;Zhang X.;Todo T. and Hara A
• 通讯作者: Todo T. and Hara A

九州北部河川における環境エストロゲン汚染の解析

九州北部河流环境雌激素污染分析

• 发表时间: 2010
• 作者: 瀬戸彩友;小柳祐美;秦拓朗;荒木一清;奈須一晃;高尾雄二;長江真樹
• 通讯作者: 長江真樹