Fabrication of anodized titanium artificial bone induced osteoblast cells

采用溶胶-凝胶法在钛板上制备了微米级图案化TiO 2薄膜。然后在含羟基磷灰石的碱性电解液中，采用直流或脉冲恒电流阳极氧化法对钛表面的图案化TiO_2凝胶膜进行阳极氧化，在图案化TiO_2膜上制备出不同形状的孔。此外，除了蚀刻工艺外，还采用光敏化和阳极氧化相结合的方法制备了反转图案膜。通过细胞培养检测，发现负载羟基磷灰石的二氧化钛膜的生物活性比不含羟基磷灰石的阳极氧化钛膜有显著提高。

Micrometer-scale patterning-TiO_2 films derived by sol-gel method were formed on a titanium plate using by photo-irradiation patterning method. The patterned TiO_2 gel film onto titanium was then anodized at constant current density with DC or pulsed-current density by anodic oxidation in an HAp-containing alkaline bath under spark discharge, to follow preparing various pore shapes on patterned-TiO_2 film. Moreover, reversed patterning-film was also prepared by the combination of photosensitizing and anodizing method except etching process. The bioactivity, which was investigated by cell culture examination, was drastically improved for HAp-loaded titania film in comparison with anodized titanium film without HAp.