Development of alkyl curdlan sulfate-coated nano fiber with specific influenza A virus-adsorptive functionality

为了开发具有流感病毒吸附功能的生物材料，通过带正电荷的双十二烷基二甲基溴化铵与带负电荷的可得然胶硫酸酯的硫酸基团之间的离子相互作用制备了可得然胶硫酸酯，并通过与可得然胶硫酸酯中长烷基的疏水作用将其涂覆在孔径为1 μm的膜过滤器上。烷基化度（DOA）为0.03（1个双十二烷基二甲基/12个含36个羟基或硫酸酯基的糖基）的烷基可得兰硫酸酯显示出强的抗HIV活性，其50%有效浓度（EC_<50>）低至0.87μg/ml（标准可得兰硫酸酯EC_<50>=0.3μg/ml）。当将1.6mg具有0.03的DOA的烷基可得然胶硫酸盐涂覆在膜滤器（13 mmΦ，孔径：1μm）上时，三个堆叠的烷基可得然胶硫酸盐涂覆的膜滤器显著地将血凝降低至1/4-1/32。因此，这些结果可被推定为证明烷基可得然胶硫酸盐涂覆的膜过滤器通过带负电荷的硫酸盐基团与病毒的带正电荷的包膜蛋白之间的吸附来去除甲型流感病毒。

To develop a biomaterial with an influenza virus-adsorptive functionality, an alkyl curdlan sulfate was prepared by ionic interaction between a positively charged didodecyldimethyl ammonium bromide and a negatively charged sulfate group of curdlan sulfate, which has potent anti-HIV activity, and then coated on a membrane filter with a 1-μm pore size by hydrophobic interaction with the long alkyl groups in the curdlan sulfate. The alkyl curdlan sulfate with the degree of alkylation (DOA) of 0.03 (1 didodecyldimethyl group/12 sugar residues with 36 hydroxyl or sulfate groups) showed potent anti-HIV activity in a 50% effective concentration (EC_<50>) as low as 0.87μg/ml (standard curdlan sulfate EC_<50>=0.3μg/ml). When 1.6 mg of the alkyl curdlan sulfate with the DOA of 0.03 was coated on a membrane filter (13 mmΦ, pore size : 1μm), three stacked alkyl curdlan sulfate-coated membrane filters dramatically decreased hemagglutination to 1/4-1/32. These results can therefore be presumed to demonstrate that the alkyl curdlan sulfate-coated membrane filter removed influenza A viruses by adsorption between the negatively charged sulfate groups and the positively charged envelope protein of the virus.