Development of TILLING platform for the elucidation of important breeding traits in tomato

开发用于阐明番茄重要育种性状的 TILLING 平台

• 批准号: 21580004
• 负责人: EZURA Hiroshi
• 金额: $ 3.08万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580004/
• 关键词: トマト; EMS; 変異体; TILLING; 重要形質; 日持ち性; 重要形

TILLING (Targeting Induced Local Lesions In Genomes) platform has been established in Micro-Tom cultivar, which is a model tomato cultivar for fruit biology. TILLING allows us to isolate mutants, which has mutations in gene of interests. Using the TILLING platform, ripening-related mutants were isolated and characterized, demonstrating the validity of the established platform.

TILLING（Targeting Induced Local Lesions In Genomes）平台已在水果生物学模式番茄品种Micro-Tom中建立。TILLING允许我们分离突变体，其在感兴趣的基因中具有突变。使用TILLING平台，成熟相关突变体的分离和表征，证明了所建立的平台的有效性。

项目成果

Tomato TILLING technology: development of a reverse genetics tool for the efficient isolation of mutants from Micro-Tom mutant libraries.

• DOI: 10.1093/pcp/pcr134
• 发表时间: 2011-11
• 期刊: Plant & cell physiology
• 影响因子: 4.9
• 作者: Okabe Y;Asamizu E;Saito T;Matsukura C;Ariizumi T;Brès C;Rothan C;Mizoguchi T;Ezura H
• 通讯作者: Ezura H

Tomato ethylene receptor mutants sletrl-1 and sletrl-2 exhibit different levels of ethylene insensitivity and impaired fruit ripening phenotype

番茄乙烯受体突变体sletrl-1和sletrl-2表现出不同水平的乙烯不敏感性和果实成熟表型受损

• 发表时间: 2012
• 作者: Yoshihiro Okabe;Erika Asamizu;Tohru Ariizumi;Hiroshi Ezura
• 通讯作者: Hiroshi Ezura

Micro-Tom TILLING technology : A reverse genetic tool for the elucidation of important traits in tomato

Micro-Tom TILLING 技术：用于阐明番茄重要性状的反向遗传工具

• 发表时间: 2012
• 作者: Hiroshi Ezura;Yoshihiro Okabe
• 通讯作者: Yoshihiro Okabe

Availability of Micro-Tom mutant library combined with TILLING in molecular breeding of tomato fruit shelf-life.

• DOI: 10.1270/jsbbs.62.202
• 发表时间: 2012-06
• 期刊: Breeding science
• 影响因子: 2.4
• 作者: Okabe Y;Asamizu E;Ariizumi T;Shirasawa K;Tabata S;Ezura H
• 通讯作者: Ezura H

Isolation and characterization of Sletr1 mutant alleles exhibiting different levels of ethylene insensitivity and impaired fruit ripening phenotype

表现出不同水平乙烯不敏感性和果实成熟受损表型的 Sletr1 突变等位基因的分离和表征

• 发表时间: 2011
• 作者: Okabe Y;Asamizu E;Ariizumi T;Ezura H.
• 通讯作者: Ezura H.