Identification of glycosyltransferases that regulate morphology and differentiation of filamentous fungus

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Aspergillus nidulans possesses three putativeα-1, 2-mannosyltransferases(Mnt) that are involved in protein O-glycosylation. We attempted to identify and characterize three Mnts of A. nidulans. The structure of O-glycans and mannosyltransferase activity using wt strain and three mnt-disruptants were determined. A. nidulans mnts(Anmnts) respectively expressed in a triple mnt mutant of yeast. The results indicate that AnMntA and AnMntC are not involved in O-glycosylation unlike to yeast Mnts, and that AnMntB functions in part similar to yeast Mnts. We determined whether AnMnts catalyze the synthetic reaction of the cell wall component, galactomannan that is present in Aspergilli. The results indicate that three AnMnts may be involved in synthesis ofα-1, 2-mannan polymer in galactomannan.