Glycomic approaches for the identification of tumor-associated glycoproteins as potential tumor markers

鉴定肿瘤相关糖蛋白作为潜在肿瘤标志物的糖组学方法

• 批准号: 21590056
• 负责人: KAWASAKI Nana
• 金额: $ 3万
• 依托单位: National Institute of Health Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590056/
• 关键词: 癌; 糖鎖; グライコミクス; プロテオミクス

Previously we suggested that heterogeneous nuclear ribonucleoproteins(hnRNPs) could be carrier proteins of sialyl Lewis x(sLe^x) glycoepitope which is widely used as a tumor marker. In this study we demonstrated that some hnRNPs were co-localized with sLe^x in Golgi apparatus, and their levels were up-regulated during S/G2 phases of the cell cycles. Furthermore, we found that expression of sLe^x was up-regulated by Fuc-T VI, and inhibition of Fuc-T VI expression suppressed the proliferation of colorectal cancer cell lines. These results suggested that sLe^x and hnRNP might be associated with the proliferation of colorectal cells and some hnRNPs could be used as novel tumor makers.

异质核核糖核蛋白（hnRNPs）可能是被广泛用作肿瘤标志物的唾液酰Lewis x（sLe^x）糖表位的载体蛋白。在这项研究中，我们证明了一些hnRNPs与sLe^x在高尔基体中共定位，并且它们的水平在细胞周期的S/G2期上调。此外，我们发现f - t VI上调sLe^x的表达，抑制f - t VI表达可抑制结直肠癌细胞系的增殖。这些结果提示sLe^x和hnRNP可能与结直肠细胞的增殖有关，一些hnRNP可能被用作新的肿瘤制造者。

项目成果

The status of α1-3/4 fucosylation at Asn 241 of β-haptoglobin is associated with colon cancer progression : A top-down and bottom-up combinatorial approach

β-触珠蛋白 Asn 241 的 α1-3/4 岩藻糖基化状态与结肠癌进展相关：自上而下和自下而上的组合方法

• 发表时间: 2012
• 期刊: Int.J.Cancer.
• 作者: Seung-Yeol Park;Sung-Hyeon Lee;Keunsoo Kang;Nana Kawasaki;Satsuki Itoh;Noritaka Hashii;Jin-Man Kim;Ji-Yeon Kim;Seon-Joo Yoon;Jung Hoe Kim
• 通讯作者: Jung Hoe Kim

創薬におけるグライコミクスアプローチ

药物发现中的糖组学方法

• 发表时间: 2011
• 作者: 川崎ナナ;橋井則貴;伊藤さつき;中澤志織
• 通讯作者: 中澤志織

The status ofα1-3/4 fucosylation at Asn 241 ofβ-haptoglobin is associated with colon cancer progression : A top-down and bottom-up combinatorial approach

β-触珠蛋白 Asn 241 的 α1-3/4 岩藻糖基化状态与结肠癌进展相关：自上而下和自下而上的组合方法

• 发表时间: 2012
• 期刊: Int. J. Cancer
• 作者: Seung-Yeol Park;Sung-Hyeon Lee;Keunsoo Kang;Nana Kawasaki;Satsuki Itoh;Noritaka Hashii;Jin-Man Kim;Ji-Yeon Kim;Seon-Joo Yoon;and Jung Hoe Kim
• 通讯作者: and Jung Hoe Kim

糖鎖関連医薬品の開発と分析化学

糖链相关药物的开发和分析化学

• 发表时间: 2010
• 期刊: ぶんせき
• 作者: Seung-Yeol Park;Sung-Hyeon Lee;Keunsoo Kang;Nana Kawasaki;Satsuki Itoh;Noritaka Hashii;Jin-Man Kim;Ji-Yeon Kim;Seon-Joo Yoon;Jung Hoe Kim;川崎ナナ
• 通讯作者: 川崎ナナ

Identification of glycoproteins carrying a target glycan-motif by liquid chromatography/multiple-stage mass spectrometry. Identification of Lewis x-glycoproteins in mouse kidney.

通过液相色谱/多级质谱法鉴定携带目标聚糖基序的糖蛋白。

• 发表时间: 2009
• 期刊: J.Proteome Res. 8
• 作者: Noritaka Hashii;Nana Kawasaki;Satsuki ltoh;Yukari Nakajima;Akira Harazono;Toru Kawanishi;Teruhide Yamaguchi
• 通讯作者: Teruhide Yamaguchi