mitochondrial multipotent functions regulated by estrogen receptor

我们使用线粒体载体（pEYFP-Mito）和小干扰RNA（siRNA）来敲除转染到小鼠睾丸的er β，研究了与线粒体及其成分分布的相关性，包括er β的定位。线粒体蛋白和er β与转染pEYFP-Mito的细胞相同。ERβsiRNA检测结果显示，er β的减少诱导Bax阳性细胞数量增加。线粒体位置的改变可能与生殖细胞凋亡有关，可能受雌激素通过ERβ调控。

We investigated the correlation with the distribution of mitochondria and their components including ERβlocalization using a mitochondria vector(pEYFP-Mito), and small interfering RNA(siRNA) to knock down ERβtransfected into mouse testes. Mitochondrial proteins and ERβwere identical to pEYFP-Mito transfected cells. ERβsiRNA assay revealed that the reduction of ERβinduced the increased number of Bax positive cells. The change of mitochondrial location may be associated with germ cell apoptosis, potentially regulated by estrogen through ERβ.