Genetic analysis of the Sec-dependent class IIa-type enterococcal bacteriocins.
Genetic analysis of the Sec-dependent class IIa-type enterococcal bacteriocins.
批准号:
21590476
负责人:
TOMITA Haruyoshi
金额:
$3.0万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011
中文摘要
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英文摘要
Genetic analysis of Bac31 and Bac43, which are the Sec-dependent class IIa-type enterococcal bacteriocins, were performed. The chimera mutants composed of two bacteriocins were constructed and analyzed. Two amino acid residues positioned at 13th and 24th were significance for the activities. The factors concerning the expression and secretion of bacteriocin were as follows, gene copy number, promoter activity, signal peptide, and host bacteria. Of the factors, the signal peptide was the most crucial for the secretion level of bacteriocin. The immunity (resistance) genes for Bac31 and Bac43 were confirmed using a newly-constructed gene-expression controllable plasmid vector named pHT7343A.
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Genetic analysis of the bactericoin 41 encoded on the Enterococcus faecalis pheromone-responsive conjugative plasmid pYI14.
对粪肠球菌信息素响应接合质粒 pYI14 上编码的细菌素 41 进行遗传分析。
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Tomita H, Todokoro D, Ike Y]
通讯作者:
Ike Y
バンコマイシン耐性腸球菌VREの高頻度接合伝達性プラスミド
用于耐万古霉素肠球菌 VRE 的高度接合传播质粒
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[Takeshi Shimizu, Yuko Ohta Masatoshi Noda,, 富田治芳]
通讯作者:
富田治芳
Genetic analysis of Enterococcus vancomycin-resistance conjugative plasmid pHTβ
肠球菌耐万古霉素接合质粒pHTβ的遗传分析
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[富田治芳, 池康嘉, Tomita H and Ike Y]
通讯作者:
Tomita H and Ike Y
Characterization of the vancomycin-resistant enterococci isolated from human patients and livestock in Taiwan : high incidence of multiple-drug resistant pheromone responsive plasmids and evidence of transmission of VRE from livestock to human.
台湾从人类患者和牲畜中分离出的耐万古霉素肠球菌的特征:多重耐药信息素响应质粒的高发生率以及万古霉素从牲畜传播到人类的证据。
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Tomita H, Lu J, Ike Y]
通讯作者:
Ike Y
Comparative analysis of the sec-dependent class IIa bacteriocins of enterococci and construction of the conditional expression vector plasmid pHT7343A
肠球菌sec依赖性IIa类细菌素的比较分析及条件表达载体质粒pHT7343A的构建
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[富田治芳, Clewell DB, 池康嘉]
通讯作者:
池康嘉
共 7 条
Analysis of the bacteriocins of vancomycin-resistant Enterococcus faecium (VRE) clinical isolates
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批准号:17590384
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.3万
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财政年份:2005
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负责人:TOMITA Haruyoshi
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依托单位:
海外基金