Identification of novel atopic dermatitis biomarkers by lipidomic analysis

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Sphingolipid metabolites such as ceramides, major lipids of the stratum corneum, are known to play critical roles in various epidermal functions. Patients with atopic dermatitis commonly show reduced ceramide contents of the skin.We developed several mass spectrometry methods(LC-MS/MS, MALDI-TOF, and LC/MS-IT-TOF) for the rapid, simultaneous quantification of C_<16>-, C_<18>-, C_<20>-, C_<22>-, C_<24>-, and C_<24>:_<1>-ceramide. This method was successfully applied to monitor the profiles of fatty acid lengths of ceramides in the stratum corneum. The profiles of fatty acid lengths of ceramides could be useful for the diagnosis of atopic dermatitis.