Elucidation of molecular mechanisms of drug-resistant leukemia through micro RNA analysis : applications to laboratory diagnostics

通过微小RNA分析阐明耐药性白血病的分子机制：在实验室诊断中的应用

• 批准号: 21590640
• 负责人: MIYACHI Hayato
• 金额: $ 3万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590640/
• 关键词: 白血病; 抗癌剤; 耐性; マイクロRNA; 抗がん剤; FLT-3; Ara-C; 抗癌剤耐; FLT-3-I; 白血病細胞; 抗癌剤耐性; FLT3-ITD

Expression of numerous genes is altered in drug-resistant tumor cells. MicroRNA is known as a regulating factor in the gene expression. The aim of this study was to clarify roles of micro RNA expression in drug-resistant leukemia cells, and its possible application in the diagnosis of resistance. When leukemia cells were transfected with FLT3-ITD, a major poor risk factor, specific resistance to ara-C was found. Altered pattern of micro RNA was demonstrated when analyzed by DNA microarray. This experimental system can be used as a model for diagnosis of drug resistance.

许多基因的表达在耐药肿瘤细胞中发生改变。MicroRNA是基因表达的调控因子。本研究的目的是阐明微小RNA在白血病耐药细胞中的作用及其在耐药诊断中的可能应用。当白血病细胞转染FLT 3-ITD（一种主要的不良风险因子）时，发现对阿糖胞苷的特异性耐药。该实验系统可作为耐药诊断的模型。

项目成果

Companion Diagnostics:臨床応用の意義と仮題

伴随诊断：临床应用意义及暂定名称

• 发表时间: 2011
• 期刊: 臨床病理
• 作者: Obayashi K;Hornsten R;Wiklund U;Karlsson M;Okamoto S;Ando Y;Suhr OB;小崎健次郎;Okuda Y;菊池正二郎;宮地勇人
• 通讯作者: 宮地勇人

Persistence of derivetive chromosome 22 after achieving a major molecular response in chronic myeloid leukemia with a cryptic BCR-ABL1 fusion gene.

使用隐秘的 BCR-ABL1 融合基因在慢性粒细胞白血病中实现主要分子反应后，衍生染色体 22 的持续存在。

• 发表时间: 2009
• 期刊: Int J Hematol 90
• 作者: Matsushita H;et al.
• 通讯作者: et al.

The Tortoiseshell Pattern in One or Both Sides of the Submandibular Glands in Mucosa‐Associated Lymphoid Tissue Lymphoma Is Related to Chromosomal Aberrations and the Disease Extent

• DOI: 10.7863/jum.2010.29.1.111
• 发表时间: 2010-01
• 期刊: Journal of Ultrasound in Medicine
• 影响因子: 2.3
• 作者: S. Asai;K. Okami;N. Nakamura;Y. Ogawa;Y. Ohta;Yoko Ogase;Guilan Jin;H. Matsushita;H. Miyachi

A novel aberrant form of e13a2 BCR-ABL1 transcript in chronic myelogenous leukemia undetectable with the standardized real-time quantitative polymerase chain reaction from the Europe Against Cancer Program

慢性粒细胞白血病中 e13a2 BCR-ABL1 转录本的一种新型异常形式，无法通过欧洲抗癌计划的标准化实时定量聚合酶链反应检测到

• 发表时间: 2009
• 期刊: Clinical Chemistry and Laboratory Medicine 47
• 作者: Matsushita H;Yamamoto M;Tsuboi K;Masukawa A;Arakawa S;Asai S;Ogawa Y;Ando K;Miyachi H
• 通讯作者: Miyachi H

A novel abberant form of e13a2 BCR-ABL1 transcript in chronic myelogenous leukemia undetectable with standardized real-time quantitative polymerase

标准化实时定量聚合酶无法检测到慢性粒细胞白血病中 e13a2 BCR-ABL1 转录物的新型异常形式

• 发表时间: 2009
• 期刊: Clin Chem Lab Med 47
• 作者: Matsushita H;et al
• 通讯作者: et al