The study on the developmental neurotoxicity of organotin in rats by analyzing mRNA and protein expressions and behaviors

通过mRNA和蛋白表达及行为分析有机锡对大鼠发育神经毒性的研究

• 批准号: 21590661
• 负责人: TSUNODA Masashi
• 金额: $ 3万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590661/
• 关键词: トリブチルスズ; 発達神経毒性; 二世代曝露; 行動学試験; 遺伝子解析; 神経毒性; 免疫毒性; オープンフィールド試験; マイクロアレイ; リアルタイムPCR

The neurotoxic effects of tributyltin(TBT) on the F1 rats were examined. The pregnant rats were exposed to TBT via their food, therefore, the F1 rats were exposed to TBT via the placenta and their dam's milk. After weaning, the half of F1 rats were exposed to TBT via their food. The inhibitory effects of behaviors in the F1 rats exposed to TBT via the placenta and their dam's milk were observed as well as the decrease in their body weight compared to the control. The F1 rats exposed to TBT via their food after weaning(at 3 weeks of age) also showed lower activities and body weight compared to the control. The exposure to TBT just after the weaning is toxic as the exposure via the placenta and their dam's milk. The inhibitory effects on the behaviors by TBT may be related to dopamine metabolism, since tyrosine hydorxylase, dopamine transporter and dopamine receptor D2 in the midbrain were down-regulated.

观察了三丁基锡（TBT）对F1代大鼠的神经毒性作用。妊娠大鼠通过食物接触三丁基锡化合物，因此，F1代大鼠通过胎盘和母鼠乳汁接触三丁基锡化合物。断奶后，一半的F1大鼠通过食物暴露于三丁基锡化合物。观察到通过胎盘和母鼠乳汁接触三丁基锡化合物的F1代大鼠的行为受到抑制，与对照组相比，体重也有所下降。与对照组相比，断奶后（3周龄）通过食物接触三丁基锡化合物的F1代大鼠的活动和体重也有所下降。断奶后立即接触三丁基锡化合物与通过胎盘和母奶接触三丁基锡化合物一样具有毒性。TBT对大鼠行为的抑制作用可能与多巴胺代谢有关，中脑酪氨酸羟化酶、多巴胺转运体和多巴胺受体D2表达下调。

项目成果

Higher toxicity of dibutyltin and poly-L-lactide with a large amount of tin but lower toxicity of poly-L-lacitide of synthetic artificial dura mater exhibited on mutine astrocyte cell line

二丁基锡和大量锡的聚-L-丙交酯对突变星形胶质细胞系的毒性较高，而合成人工硬脑膜的聚-L-丙交酯的毒性较低

• 发表时间: 2010
• 期刊: YAKUGAKU ZASSHI
• 影响因子: 0.3
• 作者: Tsuji;M.;Tsunoda.M.;et al.
• 通讯作者: et al.

Exposure to tributyltin (TBT) via food during developmental stages after weaning, or the exposure via the placenta and their dams’ milk, inhibits body weight gain and behaviors in rats.

在断奶后的发育阶段通过食物接触三丁基锡 (TBT)，或通过胎盘和母乳接触三丁基锡 (TBT)，会抑制大鼠的体重增加和行为。

• 发表时间: 2012
• 期刊: The Kitasato Medical Journal
• 作者: Ikeuchi;R.;Kido;T.;Sugaya;C.;Katagiri;H.;Akita;H.;Saji;M.;Tsunoda;M. and Aizawa;Y.
• 通讯作者: Y.

Enhanced Inhibitory Effects of TBT Chloride on the Development of F1 Rats

• DOI: 10.1007/s00244-009-9421-9
• 发表时间: 2010-05-01
• 期刊: ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
• 影响因子: 4
• 作者: Asakawa, H.;Tsunoda, M.;Aizawa, Y.
• 通讯作者: Aizawa, Y.

Evaluation of neurotoxicity of artificial dura mater containing a high concentration of dibutyltin in rats after intracranial implantation

高浓度二丁基锡人工硬脑膜颅内植入大鼠神经毒性评价

• 发表时间: 2012
• 期刊: The Kitasato Medical Journal
• 作者: Tsunoda;M.;Ikeuchi;R.;Tsuji;M.;Inoue;Y.;Ito;K.;Katagiri;H.;Akita;H.;Saji;M.;Yuba;T.;Yamada;T.;Tsuchiya;T. and Aizawa;Y
• 通讯作者: Y

トリブチルスズ二世代曝露によるF1ラットの行動への影響について～6週令での検討.

检查两代暴露于三丁基锡对 6 周龄 F1 大鼠行为的影响。

• 发表时间: 2009
• 作者: 角田正史、工藤雄一朗、田代朋子;他
• 通讯作者: 他