Analysis on molecular mechanisms of liver reconstitution by transplanted hepatic cells

本课题研究人员通过细胞表面标记物纯化小鼠原代肝干/祖细胞，对其靶分子的表达进行调控，并对移植细胞再生肝脏的分子机制进行分析。我们发现CD13是肝干/祖细胞富集的有用标记物。我们还发现sall4介导的信号通路调节肝干/祖细胞的胆道分化。我们的数据表明，MMP的调节增加了供体细胞对受体肝脏的再生。这些研究促进了自体干细胞治疗肝脏疾病的进展。

Investigators of this research project have modulated the expression of target molecules of primary murine hepatic stem/progenitor cells purified by cell surface markers, and analyzed on the molecular mechanisms of liver repopulation by transplanted cells. We found that CD13 is a useful marker for enrichment of hepatic stem/progenitor cells. We also found that Sall4-mediated signaling pathway regulates the biliary differentiation of hepatic stem/progenitor cells. Our data indicated that modulation of MMP increases to the repopulation of recipient livers by donor cells. These studies contribute to the progress of autologous stem cell therapy against liver diseases.