The signaling network between endothelium and myocardium responsible for myocardial protection

内皮和心肌之间负责心肌保护的信号网络

• 批准号: 21590930
• 负责人: NAKAOKA Yoshikazu
• 金额: $ 3.08万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590930/
• 关键词: angiopoietin-1; 冠血管; 冠静脈; 心筋; 内皮; パラクラインシグナル; neuregulin-1; EphB4; 静動洞; 静脈分化; Neuregulin-1; 心不全; Tie2; 心筋-内皮細胞間相互作用; 心内膜内皮; 心外膜; ErbB

To reveal the role of angiopoietin-1(Ang1) secreted from myocardium, we crossed Ang1flox mice withα-MHC-Cre transgenic mice to create cardiomyocyte-speicific Ang1 knockout(Ang1 CKO) mice. Ang1 CKO mice showed embryonic lethality between E12. 5 and 14. 5 with defects in(1) attachment between endocardial endothelium and ventricular traveculae,(2) development of both traveculae and compact layer, and(3) coronary vessel formation. We performed immunohistochemical analysis using CD31 antibody and found that Ang1 CKO mice exhibit the defects in coronary vein formation, namely' coronary venogenesis'.

为了揭示心肌细胞分泌的血管生成素-1（Ang 1）的作用，我们将Ang 1flox小鼠与α-MHC-Cre转基因小鼠杂交，建立心肌细胞特异性Ang 1基因敲除（Ang 1 CKO）小鼠。Ang 1 CKO小鼠在E12之间显示胚胎致死性。5和14。5例有以下缺陷：（1）内皮细胞与室间隔的附着，（2）室间隔和致密层的发育，（3）冠状血管的形成。我们使用CD 31抗体进行免疫组织化学分析，发现Ang 1 CKO小鼠表现出冠状静脉形成的缺陷，即“冠状静脉生成”。

项目成果

Gab1 is essential for postnatal angiogenesis via hepatocyte growth factor/c-Met signaling.

Gab1 通过肝细胞生长因子/c-Met 信号传导对于出生后血管生成至关重要。

• 发表时间: 2011
• 期刊: Circulation Research
• 影响因子: 20.1
• 作者: Shioyama W;Nakaoka Y;Higuchi K;Minami T;Taniyama Y;Nishida K;Kidoya H;Sonobe T;Naito H;Arita Y;Hashimoto T;Kuroda T;Fujio Y;Shirai M;Takakura N;Morishita R;Yamauchi-Takihara K;Kodama T;Hirano T;Mochizuki N;Komuro I (co-corresponding au
• 通讯作者: Komuro I (co-corresponding au

SHP2 mediates gp130-dependent cardiomyocyte hypertrophy via negative regulation of skeletalα-actin gene

SHP2通过骨骼α-肌动蛋白基因的负调节介导gp130依赖性心肌细胞肥大

• 发表时间: 2010
• 期刊: J Mol Cell Cardiol
• 影响因子: 5
• 作者: Taniguchi T;Ogata T;Naito D;Nakanishi N;Miyagawa K;Amano K;Isodono K;Imoto H;Tagawa M;Takehara N;Asada S;Adachi A;Morimoto T;Takahashi T;Oh H;Ueyama T;Matsubara H;Nakaoka Y
• 通讯作者: Nakaoka Y

Scaffolding Docking Protein Gab1 Has an Essential Role for HGF-depen dent Endothelial Signaling and Angiogenesis after Hindlimb Ischemia

支架对接蛋白 Gab1 对后肢缺血后 HGF 依赖性内皮信号传导和血管生成具有重要作用

• 发表时间: 2009
• 作者: Nakaoka Y.;et al.
• 通讯作者: et al.

The Role of Gab Familyl Docking Proteins in the Pathophysiology of the Cardiovascular Diseases

Gab 家族对接蛋白在心血管疾病病理生理学中的作用

• 发表时间: 2011
• 作者: Nakaoka Y;Higuchi K;Shioyama W;Taniyama Y;Arita Y;Hashimoto T;Fujio Y;Yamauchi-Takihara K;Morishita R;Hirano T;Komuro I;Sugihara K;西山功一;平野勝也;Nakaoka Y
• 通讯作者: Nakaoka Y

A case of non-cardiogenic acute pulmonary edema in a patient with POEMS syndrome-associated pulmonary arterial hypertension

POEMS综合征相关肺动脉高压患者发生非心源性急性肺水肿1例

• 发表时间: 2011
• 期刊: Ann Hematol
• 影响因子: 3.5
• 作者: Hara M;Mizote I;Nakaoka Y;Tanaka H;Asano Y;Sakata Y;Komuro I
• 通讯作者: Komuro I