Isolation of erythropoietin-producing cells using embryonic stem cells

使用胚胎干细胞分离促红细胞生成素产生细胞

• 批准号: 21591037
• 负责人: KUBO Atsushi
• 金额: $ 3.08万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591037/
• 关键词: ES細胞; エリスロポエチン; 腎臓; 胚性幹細胞

1. Targeting vector was designed to integrate EGFP in erythropoietin(EPO) gene. EGFP-IRES-puroR gene was knocked-in into the start codon of EPO gene. This vector was electroprated into C57BL/6 derived ES cells and then EPO-EGFP ES cells were established. These ES cells were cultured in various condition such as ectoderm, mesoderm and endoderm and EGFP was evaluated by FACS.2. EPO-EGFP ES cells were injected into mouse blastcyst and then EPO-EGFP+/-mouse was established. Section of kidney from EPO-EGFP+/-was analyzed by fluorescent microscopy. Isolated cells from EPO-EGFP+/-mouse were analyzed by FACS.

1. 设计了EGFP整合促红细胞生成素（EPO）基因的靶向载体。EGFP-IRES-puroR基因被敲入EPO基因的起始密码子。将该载体电沉积于C57BL/6衍生的胚胎干细胞中，构建EPO-EGFP胚胎干细胞。将这些胚胎干细胞分别培养于外胚层、中胚层和内胚层等不同条件下，用流式细胞法测定EGFP。将EPO-EGFP ES细胞注入小鼠胚泡，建立EPO-EGFP+/-小鼠。用荧光显微镜分析肾EPO-EGFP+/-切片。用流式细胞仪对EPO-EGFP+/-小鼠分离细胞进行分析。

项目成果

Epithelial-Mesenchymal Transition as a Potential Explanation for Podocyte Depletion in Diabetic Nephropathy

• DOI: 10.1053/j.ajkd.2009.05.009
• 发表时间: 2009-10-01
• 期刊: AMERICAN JOURNAL OF KIDNEY DISEASES
• 影响因子: 13.2
• 作者: Yamaguchi, Yukinari;Iwano, Masayuki;Saito, Yoshihiko
• 通讯作者: Saito, Yoshihiko

The Homeobox Gene Hex Regulates Hepatocyte Differentiation from Embryonic Stem Cell-Derived Endoderm

• DOI: 10.1002/hep.23293
• 发表时间: 2010-02-01
• 期刊: HEPATOLOGY
• 影响因子: 13.5
• 作者: Kubo, Atsushi;Kim, Yon Hui;Keller, Gordon
• 通讯作者: Keller, Gordon

Treatment with Recombinant Placental Growth Factor (PlGF) Enhances both Angiogenesis and Arteriogenesis and Improves Survival after Myocardial Infarction

重组胎盘生长因子 (PlGF) 治疗可增强血管生成和动脉生成，并提高心肌梗塞后的生存率

• 发表时间: 2009
• 期刊: PLGF therapy in acute myocardial infarction. Circ J (in press)
• 作者: Takeda Y;Uemura S;Saito Y;(and15 authors)
• 通讯作者: (and15 authors)

Possible involvement of stem-like populations with elevated ALDH1 in sarcomas for chemotherapeutic drug resistance

• DOI: 10.3892/or_00000885
• 发表时间: 2010-08-01
• 期刊: ONCOLOGY REPORTS
• 影响因子: 4.2
• 作者: Honoki, Kanya;Fujii, Hiromasa;Tsujiuchi, Toshifumi
• 通讯作者: Tsujiuchi, Toshifumi