Molecular mechanism of autophagy in follicular lymphoma cells

滤泡性淋巴瘤细胞自噬的分子机制

• 批准号: 21591196
• 负责人: YOSHIDA Akira
• 金额: $ 3万
• 依托单位: University of Fukui
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591196/
• 关键词: Bcl-2; 濾胞性リンパ腫; オートファジー; LC-II; ABT-737; Beclin-1

We investigated the cell death mechanism of Bcl-2 over expressing human leukemia and lymphoma cells. We found that etoposide treatment increased Beclin-1 expression, the conversion of the soluble form of microtubule-associated protein 1 light chain 3(LC3) to the autophagic vesicle-associated form LC3-II and the occurence of lysosomes/autophagosomes in Bcl-2 over expressing cells. The autophagy inhibitor chloroquine enhanced the cytotoxicity of etoposide in Bcl-2 over expressing lymphoma cells. These data indicate that autophagy may play an important role as protective response after etoposide treatment.

我们研究了Bcl-2过表达的人白血病和淋巴瘤细胞的细胞死亡机制。我们发现，依托泊苷治疗增加Beclin-1的表达，微管相关蛋白1轻链3（LC 3）的可溶性形式的自噬囊泡相关的形式LC 3-II和溶酶体/自噬体的发生在Bcl-2过表达细胞的转换。自噬抑制剂氯喹增强了依托泊苷对Bcl-2过表达淋巴瘤细胞的细胞毒性。这些数据表明，自噬可能发挥重要作用，作为保护性反应后，足叶乙甙治疗。

项目成果

Camptothecin induces DNA strand breaks and is cytotoxic in stimulated normal lymphocytes

喜树碱会诱导 DNA 链断裂，并对受刺激的正常淋巴细胞具有细胞毒性

• DOI: 10.3892/or.2010.1100
• 发表时间: 2011
• 期刊: Oncol Rep
• 影响因子: 4.2
• 作者: Yamauchi T;Yoshida A;Ueda T
• 通讯作者: Ueda T

Marked Upregulations of Survivin and Aurora-B Kinase Are Associated with Disease Progression in the Myelodysplastic Syndromes

Survivin 和 Aurora-B 激酶的显着上调与骨髓增生异常综合征的疾病进展相关

• 发表时间: 2011
• 作者: Yoshida;A. Zokumasu K.;Yamauchi;T.;Takagi;K.;Kishi;S.;Urasaki;Y.;Tohyama;K.;Ueda T.
• 通讯作者: Ueda T.

Marked upregulation of Survivin and Aurora-B kinase are associated with disease progression in the myelodysplastic syndromes

Survivin 和 Aurora-B 激酶的显着上调与骨髓增生异常综合征的疾病进展相关

• 发表时间: 2012
• 期刊: Haematologica
• 影响因子: 10.1
• 作者: Yoshida A;Zokumasu K;Wano Y;Yamauchi T;他
• 通讯作者: 他

Yoshida, A.Zokumasu K., Yamauchi, T., Takagi, K., Kishi, S., Urasaki, Y., Tohyama, K., Ueda T. : Marked Upregulations of Survivin and Aurora-B Kinase Are Associated with Disease Progression in the Myelodysplastic Syndromes

Yoshida, A.Zokumasu K.、Yamauchi, T.、Takagi, K.、Kishi, S.、Urasaki, Y.、Tohyama, K.、Ueda T.：生存素和 Aurora-B 激酶的显着上调与疾病相关

• 发表时间: 2011
• 作者: Yoshida A;Ueda T
• 通讯作者: Ueda T

The indolocarbazole derivative Go6976 suppresses the growth of leukemia cells with FLT3 mutations

吲哚并咔唑衍生物Go6976抑制FLT3突变的白血病细胞的生长

• 发表时间: 2012
• 作者: Yoshida;A;Zokumasu;k.;Ueda T
• 通讯作者: Ueda T