Effect of DNA methylation on the regulation of microRNA in squamous cell carcinoma of the esophagus

DNA甲基化对食管鳞癌microRNA调控的影响

• 批准号: 21591704
• 负责人: WATANABE Masayuki
• 金额: $ 2.91万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591704/
• 关键词: DNAメチル化異常; SYBR green法; microRNA; ホルマリン固定標本; 化学療法感受性; 食道癌; 食道扁平上皮癌; micoRNA; pyrosequence; LINE-1; FBXW7; ZEB-2

We clarified that global hypomethylation was observed in squamous cell carcinoma of the esophagus(ESCC) and the hypomethylation of LINE-1 was a significant indicator for poor prognosis in patients with ESCC. We also identified that overexpression of microRNA(miR)-29c was a prognostic factor of patients with ESCC and miR-223 regulated FBXW7 in ESCC. We examined miR-21 expression in the serum and clarified that the serum miR-21 levels of ESCC patients was significantly higher than those of healthy controls and the serum miR-21 expression could be a marker for treatment effect for ESCC.

我们阐明了在食管鳞状细胞癌（ESCC）中观察到的整体低甲基化，LINE-1的低甲基化是ESCC患者预后不良的重要指标。我们还发现microRNA（miR）-29c的过表达是ESCC患者的预后因素，并且miR-223在ESCC中调节FBXW 7。我们检测了血清中miR-21的表达，并阐明了ESCC患者血清中miR-21的水平显著高于健康对照，血清miR-21的表达可以作为ESCC治疗效果的标志。

项目成果

Serum microRNA-21 is a novel biomarker in patients with esophageal squamous cell carcinoma

• DOI: 10.1002/jso.23064
• 发表时间: 2012-08-01
• 期刊: JOURNAL OF SURGICAL ONCOLOGY
• 影响因子: 2.5
• 作者: Kurashige, Junji;Kamohara, Hidenobu;Baba, Hideo
• 通讯作者: Baba, Hideo

LINE-1 Hypomethylation Is Associated With a Poor Prognosis Among Patients With Curatively Resected Esophageal Squamous Cell Carcinoma

• DOI: 10.1097/sla.0b013e31826d8602
• 发表时间: 2013-03-01
• 期刊: ANNALS OF SURGERY
• 影响因子: 9
• 作者: Iwagami, Shiro;Baba, Yoshifumi;Baba, Hideo
• 通讯作者: Baba, Hideo

胃癌におけるmicroRNA-200bによる癌浸潤転移の制御機構 / The miR-200b regulates epithelial to mesenchymal transition in gastric cancer

miR-200b 调节胃癌上皮间质转化

• 发表时间: 2011
• 作者: Kinoshita H;Okabe H;Beppu T;Chikamoto A;Hayashi H;Imai K;Mima K;Nakagawa S;Yokoyama N;Ishiko T;Shinriki S;Jono H;Ando Y;Baba H.;蔵重淳二
• 通讯作者: 蔵重淳二

MicroRNA-21 regulates proliferation and invasion in esophagus squamous cell carcinoma

MicroRNA-21调节食管鳞状细胞癌的增殖和侵袭

• 发表时间: 2009
• 作者: Kitamura A;Matsushita K;Takiguchi Y;Shimada Hideaki;Tomonaga T;Matsubara H.Inou;M;Mamoru H;Sato Y;Levens D;Tatsumi K;Nomura F.;日吉幸晴
• 通讯作者: 日吉幸晴

The Identificationof novel micro RNAs and Genes in bone marrow regulating gastric cancer metastasis

骨髓调控胃癌转移的新型微小RNA和基因的鉴定

• 发表时间: 2011
• 作者: Junji Kurashige ;Masayuki Watanabe;Yukiharu Hiyoshi ;Hidenobu Kamohara ;Koichi Kinoshita;Seiya Saito;Masaaki Iwatsuki;Yoshifumi Baba ;Naoko Hayashi ;Hideo Baba;Kurashige J;Kinoshita K;Nakahara O;Kurashige;Kurahsige J;Iwatsuki M
• 通讯作者: Iwatsuki M