The effect of suppression of NF kB activity on the radio-sensitivity of colon cancer cells and on the prevention of intestinal damage.

抑制 NF kB 活性对结肠癌细胞放射敏感性和预防肠道损伤的影响。

• 批准号: 21591738
• 负责人: YOSHIDA Yasuhiko
• 金额: $ 2.91万
• 依托单位: Hyogo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591738/
• 关键词: 放射性感受性; 肝細胞増殖因子; おとり型核酸; 放射線障害; 放躰性感受性; 放射線感受性

In a mouse model of radiation induced-chronic intestinal injury, fibrogenesis was seen in the submucosal layer. We investigated the effect of HGF administration and NF-kB decoy transfection on fibrogenesis of intestinal wall in a mouse model. HGF administration, but not NF-kB decoy transfection, inhibited the fibrogenesis in the intestinal wall. Next, we investigated the effect of low-dose HGF administration and NF-kB decoy transfection on proliferation ability of a cell line of human colon cancer. NF-kB decoy transfection, but not low-dose HGF administration, inhibited proliferation of cancer cells. Last, we investigated the relationship between NF-kB and EMT in cancer cells. Unfortunately, NF-kB transfection did not change the gene expression of EMT related molecules.

在辐射诱导的小鼠慢性肠损伤模型中，粘膜下层可见纤维生成。我们研究了HGF给药和NF-kB诱饵转染对小鼠模型肠壁纤维形成的影响。HGF处理抑制了肠壁纤维的形成，而NF-kB诱骗转染则没有作用。接下来，我们研究了低剂量HGF和NF-kB诱饵转染对人结肠癌细胞系增殖能力的影响。NF-kB诱饵转染，而不是低剂量HGF给药，抑制癌细胞的增殖。最后，我们研究了肿瘤细胞中NF-kB与EMT的关系。不幸的是，NF-kB转染并没有改变EMT相关分子的基因表达。

项目成果

Impact of preoperative 3D hepatectomy simulation system for liver resection and transplantation : Application to various kind of liver resection procedure

术前3D肝切除模拟系统对肝切除和移植的影响：在各种肝切除手术中的应用

• 发表时间: 2011
• 作者: Fujimoto J;Iimuro Y;Yada A;Kondo Y;Saito S;Sueoka H;Ohashi K.
• 通讯作者: Ohashi K.

Toll-like receptor signaling in liver regeneration, fibrosis and carcinogenesis.

• DOI: 10.1111/j.1872-034x.2011.00822.x
• 发表时间: 2011-07
• 期刊: Hepatology research : the official journal of the Japan Society of Hepatology
• 作者: Seki E;Park E;Fujimoto J
• 通讯作者: Fujimoto J

肝細胞癌内線維化領域に存在する線維芽細胞の免疫組織学的検討

肝细胞癌纤维化区域中成纤维细胞的免疫组织学检查

• 发表时间: 2010
• 作者: Iimuro Y;Kashiwagi T;Yamanaka J;Hirano T;Saito S;Sugimoto T;Watanabe S;Kuroda N;Okada T;Asano Y;Uyama N;Fujimoto J;鈴村和大,森川司朗,岡田敏弘,王孔志,黒田暢一,平野公通,宇山直樹,佐竹真,藤元治朗;宇山直樹,波多野悦朗,前谷洋爾,磯田裕義,柴田登志也,田浦康二郎,大江正士郎,内藤雅人,安近健太郎,藤井英明,猪飼伊和夫,上本伸二;宇山直樹;宇山直樹,飯室勇二,中井紀博,近藤祐一,鈴村和大,佐竹真,吉田康彦,杉本貴昭,麻野泰包,斉藤慎一,王孔志,岡田敏弘,平野公通,黒田暢一,山中潤一,藤元治朗
• 通讯作者: 宇山直樹,飯室勇二,中井紀博,近藤祐一,鈴村和大,佐竹真,吉田康彦,杉本貴昭,麻野泰包,斉藤慎一,王孔志,岡田敏弘,平野公通,黒田暢一,山中潤一,藤元治朗

In vivo effect of imatinib on progression of cecal GIST-like tumors in exon 17-type c-kit knock-in mice

伊马替尼对外显子 17 型 c-kit 敲入小鼠盲肠 GIST 样肿瘤进展的体内影响

• 发表时间: 2009
• 期刊: Lab Invest 89
• 作者: K amo N;Naomoto Y;Shirakawa Y;Yamatsuji T;Hirota S;Fujiwaraa Y;Noma K;Sakurama K;Takaoka M;Nagatsuka H;Gunduz M;Matsuoka J;Tanaka N.;Nishida T;Kamo N;Ishikawa T
• 通讯作者: Ishikawa T

Application of computational portal flow dynamics simulation to liver regeneration analysis

计算门静脉血流动力学模拟在肝再生分析中的应用

• 发表时间: 2009
• 作者: Iimuro Y;Uyama N;Sugimoto T;Saito S;Hirano T;Yamanaka J;Fujimoto J.
• 通讯作者: Fujimoto J.