New treatment strategy for Hirschsprung's disease with neural crest stem cells by tissue-engneering

为了寻找治疗巨结肠病的新策略，我们尝试利用三维培养技术将诱导多能干细胞向肠神经系统分化。我们证明了小鼠iPS细胞在体外三维培养中具有组织具有运动功能的肠样器官的能力。肠样器官由三个胚层的肠道成分组成。在本研究针对巨结肠病的治疗中，利用具有疾病特异性的iPS细胞组织包括神经系统在内的功能性肠道是非常重要的发现。

For a new treatment strategy for Hirschsprung's disease, we tried to performe the differentiation from induced pluripotent stem cells to enteric nervous system using the three-dimensional culture. We demonstrate that mouse iPS cells have the ability to organize a gut-like organ with motor function in vitro by three-dementional culture. A gut-like organ was composed of all the enteric components of three germ layers. When this study aims for treatment of Hirschsprung's disease, it was very important discovery to organization of functional gut including the nervous system from iPS cells which have a disease-specific characteristic.