Molecular mechanism to regulate the patterning of craniofacial skeleton.

调节颅面骨骼模式的分子机制。

• 批准号: 21592610
• 负责人: KAWAKAMI Masayoshi
• 金额: $ 2.91万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592610/
• 关键词: 歯科矯正学; 形態発生; 遺伝子; 細胞・組織; 発生・分化; 国際情報交換; 歯学; chick embrvo; L3/Lhx8; FGF; chick embryo; L3; Lhx8

Expression of the LIM homeodomain transcription factor Lhx8 is restricted to and up-regulated in the mesenchyme of the upper face prominence before lip fusion. Since retinoid is a potential patterning influence on the developing face, we examined the regulation of Lhx8 through retinoic acid(RA) in the maxillary prominence and detected signals that induce or repress Lhx8 expression. Application of exogenous RA caused severe defects of the maxilla. Citral also induced a specific loss of derivatives from the maxillary prominences by blocking RA synthesis. In situ hybridization and RT-PCR analysis of the maxillary mesenchyme revealed that the expression of Lhx8 was significantly down-regulated by RA as well as by Citral and FGF-8b whereas SU5402, a pan-FGF family antagonist, down-regulated the gene and caused defective maxillary morphogenesis and cleft lip. Our data suggest that Lhx8 is regulated by RA signaling through FGF signals during tissue interactions of maxillary morphogenesis.

LIM同源结构域转录因子Lhx 8的表达被限制在唇融合前的上面部突起的间充质中并在其中上调。由于类维生素A是一个潜在的图案的影响，发展中国家的脸，我们研究了通过视黄酸（RA）的调节Lhx 8在上颌突出和检测信号，诱导或抑制Lhx 8的表达。外源性RA的应用导致上颌骨严重缺损。柠檬醛还通过阻断RA的合成，诱导上颌骨的衍生物的特定损失。原位杂交和RT-PCR分析显示，Lhx 8的表达显着下调RA以及柠檬醛和FGF-8b，而SU 5402，一个泛FGF家族拮抗剂，下调该基因，并造成缺陷的上颌骨形态和唇裂。我们的数据表明，Lhx 8调节RA信号通过FGF信号在组织相互作用的上颌形态发生。

项目成果

Regulation and/or repression of cholinergic differentiation of murine embryonic stem cells using RNAi directed against transcription factor L3/Lhx8

使用针对转录因子 L3/Lhx8 的 RNAi 调节和/或抑制小鼠胚胎干细胞的胆碱能分化

• DOI: 10.1007/978-1-60761-769-3_8
• 发表时间: 2010
• 期刊: Methods in Molecular Biology
• 作者: Manabe T;Wanaka A
• 通讯作者: Wanaka A

口唇形成におけるL3/Lhx8遺伝子発現とWntシグナルの役割

L3/Lhx8 基因表达和 Wnt 信号在唇形成中的作用

• 发表时间: 2010
• 作者: 川上正良;藤井亮介;奥田洋明;和中明生;桐田忠昭
• 通讯作者: 桐田忠昭

Orthodontic rehabilitation for anterior teeth lost due to trauma with crowding malocclusion

因外伤导致前牙缺失并拥挤错牙合的正畸康复

• DOI: 10.1111/j.1600-9657.2010.00876.x
• 发表时间: 2010
• 期刊: Dental Traumatology
• 影响因子: 2.5
• 作者: Kawakami M;Okamoto K;Fujii R;Kirita T
• 通讯作者: Kirita T

口唇形成時のWntシグナリングがMsx遺伝子発現に及ぼす影響

Wnt信号对唇形成过程中Msx基因表达的影响

• 发表时间: 2011
• 作者: 藤井亮介;川上正良;奥田洋明;和中明生;桐田忠昭
• 通讯作者: 桐田忠昭

Transient activation of Notch signaling in the injured adult brain

• DOI: 10.1016/j.jchemneu.2009.09.003
• 发表时间: 2010-01-01
• 期刊: JOURNAL OF CHEMICAL NEUROANATOMY
• 影响因子: 2.8
• 作者: Tatsumi, Kouko;Okuda, Hiroaki;Wanaka, Akio
• 通讯作者: Wanaka, Akio