Single molecule analyses around the synaptic membrane formed on glass

玻璃上形成的突触膜周围的单分子分析

• 批准号: 21650073
• 负责人: HIRANO Tomoo
• 金额: $ 2.22万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21650073/
• 关键词: シナプス; グルタミン酸受容体; 全反射顕微鏡; 長期増強; 細胞培養; 培養; ニューレキシン; 一分子; ダルタミン酸受容体

Information transmission between neurons takes place at synapses. Here, we have succeeded to form postsynaptic membrane directly on the glass and to observe the movement of receptor molecules with high spatial and temporal resolution using total internal reflection fluorescent microscopy. We have demonstrated that different subtypes of glutamate receptor accumulate at the postsynaptic membrane through distinct routes during long-term potentiation, which is the neuronal activity dependent enhancement of synaptic transmission and has been regarded as a basis of learning and memory.

神经元之间的信息传递发生在突触上。在这里，我们已经成功地直接在玻璃上形成突触后膜，并使用全内反射荧光显微镜以高的空间和时间分辨率观察受体分子的运动。长时程增强是神经元活动依赖性的突触传递增强过程，是学习记忆的基础。

项目成果

Subunit-specific translocation of AMPA receptors to post-synaptic membrane during hippocampal LTP. Neuroscience 201

海马 LTP 期间 AMPA 受体亚基特异性易位至突触后膜。

• 发表时间: 2011
• 作者: Ageta-Ishihara;N.;et al.;Tanaka H & Hirano T
• 通讯作者: Tanaka H & Hirano T

Roles of glutamate receptor δ family in synapse formation.

谷氨酸受体δ家族在突触形成中的作用。

• 发表时间: 2009
• 作者: Hirano;T.
• 通讯作者: T.

Prediction and validation of a mechanism to control the threshold for inhibitory synaptic plasticity.

• DOI: 10.1038/msb.2009.39
• 发表时间: 2009
• 期刊: MOLECULAR SYSTEMS BIOLOGY
• 影响因子: 9.9
• 作者: Kitagawa, Yuichi;Hirano, Tomoo;Kawaguchi, Shin-ya
• 通讯作者: Kawaguchi, Shin-ya

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Subunit-specific translocation of AMPA receptors during hippocampal LTP

海马 LTP 期间 AMPA 受体的亚基特异性易位

• 发表时间: 2011
• 作者: Tanaka H;Hirano T;Tanaka H & Hirano T
• 通讯作者: Tanaka H & Hirano T