Development of DNA-Chip-Based Electronic Devices for Nanobio Sensing Applications

开发用于纳米生物传感应用的基于 DNA 芯片的电子设备

• 批准号: 21200034
• 负责人: NAKANO Kouji
• 金额: $ 16.14万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21200034/
• 关键词: ナノバイオ; ＤＮＡ; 自己組織化; 分子認識; バイオセンサー; 遺伝子センサー; 分子エレクトロニクス; 走査プローブ顕微鏡; DNA; 走査プローブ顕徽鏡; 走査プローズ顕微鏡

DNA-based molecular device that is capable of responding to biological molecular recognition events was tackled for futuristic nano-, bio-device developments based on in-situ electoconductive modification of DNA self-assembly. Some direct or indirect assistance necessary to the goal were successfully developed; novel psoralen compounds that enable versatile functionalization of DNA duplexes by covalent conjugation, single-molecule assay for various DNA affinity reactions including single-nucleotide polymorphism using atomic force microscope, new types of bottom-up, supramolecular DNA nanomaterials based on dendrimer formation of three-way junction DNA, and etc. We are committed to taking on the challenges by installing the DNA dendrimer materials into interdigitated array electrode to provide state-of-the-art, molecularly responsive devices adaptable to various bioanalytical purposes.

基于DNA的分子器件能够响应生物分子识别事件，是未来纳米生物器件开发的基础上，DNA自组装的原位导电改性。成功地开发了实现这一目标所需的一些直接或间接援助;本发明涉及能够通过共价缀合实现DNA双链体的多功能化的新型卟啉化合物、用于各种DNA亲和反应的单分子测定（包括使用原子力显微镜的单核苷酸多态性）、基于三向连接DNA的树枝状聚合物形成的新型自下而上的超分子DNA纳米材料我们致力于通过将DNA树枝状聚合物材料安装到叉指阵列电极中来应对挑战，以提供适用于各种生物分析目的的最先进的分子响应装置。

项目成果

Positive-feedback-mode scanning electrochemical microscopy imaging of DNA-grafted benzoqumone polymer cast film for micrometer-sized hyblidization biosensor applications

用于微米级杂交生物传感器应用的 DNA 接枝苯醌聚合物流延膜的正反馈模式扫描电化学显微镜成像

• 发表时间: 2010
• 作者: K. Nakano;K. Nakamura;T. Kimra;H. Yoshinaga;N. Soh;T. Imoto
• 通讯作者: T. Imoto

Anodic Stripping Voltammetry Combined with Sequential Injection Analysis for Measurements of Trace Metal Ions with Bismuth- and Antimony Film Electrodes under Comparable Conditions

• DOI: 10.1002/elan.201000683
• 发表时间: 2011-07-01
• 期刊: ELECTROANALYSIS
• 影响因子: 3
• 作者: Guzsvany, Valeria;Nakajima, Hizuru;Imato, Toshihiko
• 通讯作者: Imato, Toshihiko

Programmable protein-protein conjugation via DNA-based self-assembly.

• DOI: 10.1039/c2cc30618b
• 发表时间: 2012-05
• 期刊: Chemical communications
• 影响因子: 4.9
• 作者: J. Shimada;T. Maruyama;Momoko Kitaoka;H. Yoshinaga;K. Nakano;N. Kamiya;M. Goto

アルカンチオール単分子膜修飾電極に固定化したチトクロームcの直接電子移動反応の研究

烷硫醇单层修饰电极固定细胞色素c直接电子转移反应的研究

• 发表时间: 2009
• 期刊: 九州大学中央分析センター報告 27
• 作者: 中野幸二;ら
• 通讯作者: ら

Self-Assembly of Dendrimer-Like DNA and Electrical Conductivity Measurements Using Interdigitated Microelectrodes

使用叉指微电极进行类树枝状 DNA 的自组装和电导率测量

• 发表时间: 2011
• 作者: Manabu Hatano;Kazuaki Ishihara;Koji Nakano
• 通讯作者: Koji Nakano