Molecular Anatomy of Synaptic Structure

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PALM and STORM are superresolution imaging techniques based on detection of single fluorescent molecules. Although postsynaptic densities (PSDs) are disc-like microstructures with their diameters of less than 500 nm, PALM and STORM are able to resolve their internal structure. In this study, fluorescently labeled PSDs and spines were visualized by using PALM and the molecular distributions within these structures were detected. Furthermore, we could successfully detected distributional changes of PSD molecules during development of synapses and after induction of synaptic plasticity.