Cellular and molecular basis of hippocampal presynaptic calcium store

海马突触前钙储存的细胞和分子基础

• 批准号: 21300146
• 负责人: KAMIYA Haruyuki
• 金额: $ 12.4万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21300146/
• 关键词: シナプス伝達; カルシウムイオン; 海馬; 神経伝達物質放出

Subcellular localization of calcium releasing sites from presynaptic calcium store in hippocampal mossy fiber synapse was examined. Using high speed confocal microscope system, calcium imaging within mossy fiber terminals and axons was performed. Calcium indicator was efficiently introduced to single mossy fiber terminals and axons by electroporation through microelectrode placed on visually identified mossy fiber terminals.

本实验观察了突触前钙库钙释放位点在海马苔藓纤维突触的亚细胞定位。利用高速共聚焦显微镜系统，对苔藓纤维终末和轴突内钙离子进行成像。通过将微电极置于视觉识别的苔藓纤维末端，通过电穿孔将钙指示剂有效地引入到单个苔藓纤维末端和轴突。

项目成果

Bumetanide-sensitive and -insensitive actions of GABA_A receptors on the excitability of the hippocampal mossy fibers

GABA_A 受体布美他尼敏感和不敏感作用对海马苔藓纤维兴奋性的影响

• 发表时间: 2009
• 作者: Kamiya;H.
• 通讯作者: H.

光不活化法による海馬CA1野シナプスAMPA受容体動態の長期計測

利用光失活法长期测量海马 CA1 区突触 AMPA 受体动态

• 发表时间: 2011
• 作者: Yamazaki D;Horiuchi J;Miyashita T;Saitoe M.;神谷温之
• 通讯作者: 神谷温之

Photochemical inactivation of postsynaptic AMPA receptors in hippocampal slices

海马切片突触后 AMPA 受体的光化学失活

• 发表时间: 2010
• 作者: Tamura T;Horiuchi D;Chen;Y.C;Sone M;Miyashita T;Saitoe;M;Yoshimura N;Chiang A.S;Okazawa H.;金子雅幸;Kamiya H.
• 通讯作者: Kamiya H.

NMDA Receptor GluN2B (GluRε2/NR2B) Subunit Is Crucial for Channel Function, Postsynaptic Macromolecular Organization, and Actin Cytoskeleton at Hippocampal CA3 Synapses

• DOI: 10.1523/jneurosci.5531-08.2009
• 发表时间: 2009-09-02
• 期刊: JOURNAL OF NEUROSCIENCE
• 影响因子: 5.3
• 作者: Akashi, Kaori;Kakizaki, Toshikazu;Sakimura, Kenji
• 通讯作者: Sakimura, Kenji

Heterosynaptic enhancement of the excitability of hippocampal mossy fibers by long-range spill-over of glutamate

谷氨酸远程溢出对海马苔藓纤维兴奋性的异质突触增强

• DOI: 10.1002/hipo.20885
• 发表时间: 2012
• 期刊: Hippocampus
• 影响因子: 3.5
• 作者: Uchida T.;Fukuda S.;Kamiya H.
• 通讯作者: Kamiya H.