Risk assessment of environmental oxidative chemicals using catalase mutant primary cultured hepatocytes

我们将小鼠原代肝细胞暴露在日常生活中常用的化学物质中，如对苯二酚、劳松酮。我们发现，过氧化氢酶突变小鼠(Cs^b)和野生型小鼠(Cs^a)暴露于这些化学物质后，细胞存活率均呈剂量依赖性下降，尤其是Csb组肝细胞存活率低于Cs^a组。我们还观察到，皮肤美白乳膏的主要成分对苯二酚可剂量依赖性地增强细胞色素P450 2E1mRNA和蛋白的表达，并诱导细胞凋亡。

We exposured mouse primary hepatocytes to chemicals that are commonly used in our daily lives like hydroquinone, lawsone. We found that exposure the cells to these chemicals decreased cellular viability in both catalase mutant mice (Cs^b) and the wild-type mice (Cs^a) dose-dependently, in particular, the survival of hepatocytes was lower in Csbwhen compared to that of Cs^a. We also observed that exposure the cells to hydroquinone, a main component of skin-lightening cream, enhanced the cellular expression of cytochrome P450 2E1 mRNA and protein dose-dependently as well as induced apoptosis of the cells.