Risk assessment of environmental oxidative chemicals using catalase mutant primary cultured hepatocytes

我们将小鼠原代肝细胞暴露于我们日常生活中常用的化学物质，如对苯二酚，指甲松。我们发现，将细胞暴露于这些化学物质会剂量依赖性地降低过氧化氢酶突变小鼠（Cs^B）和野生型小鼠（Cs^a）的细胞活力，特别是与Cs^a相比，Cs ^b中的肝细胞存活率较低。我们还观察到，暴露于氢醌，美白霜的主要成分，细胞色素P450 2E1 mRNA和蛋白的表达增强细胞剂量依赖性，并诱导细胞凋亡。

We exposured mouse primary hepatocytes to chemicals that are commonly used in our daily lives like hydroquinone, lawsone. We found that exposure the cells to these chemicals decreased cellular viability in both catalase mutant mice (Cs^b) and the wild-type mice (Cs^a) dose-dependently, in particular, the survival of hepatocytes was lower in Csbwhen compared to that of Cs^a. We also observed that exposure the cells to hydroquinone, a main component of skin-lightening cream, enhanced the cellular expression of cytochrome P450 2E1 mRNA and protein dose-dependently as well as induced apoptosis of the cells.