Risk assessment of environmental oxidative chemicals using catalase mutant primary cultured hepatocytes

使用过氧化氢酶突变体原代培养肝细胞进行环境氧化化学物质的风险评估

• 批准号: 21310022
• 负责人: WANG Da-hong
• 金额: $ 8.9万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21310022/
• 关键词: 初代培養肝細胞; カタラーゼ; 過酸化水素; ヒドロキノン; アポトーシス; ローソン; レスベラトロール; 化学物質の生体影響評価; 生体影響評価; カタラーゼ遺伝子変異マウス; プロテオーム; 美白クリーム; 酸化ストレス; 環境影響評価

We exposured mouse primary hepatocytes to chemicals that are commonly used in our daily lives like hydroquinone, lawsone. We found that exposure the cells to these chemicals decreased cellular viability in both catalase mutant mice (Cs^b) and the wild-type mice (Cs^a) dose-dependently, in particular, the survival of hepatocytes was lower in Csbwhen compared to that of Cs^a. We also observed that exposure the cells to hydroquinone, a main component of skin-lightening cream, enhanced the cellular expression of cytochrome P450 2E1 mRNA and protein dose-dependently as well as induced apoptosis of the cells.

我们将小鼠原代肝细胞暴露在日常生活中常用的化学物质中，如对苯二酚、劳松酮。我们发现，过氧化氢酶突变小鼠(Cs^b)和野生型小鼠(Cs^a)暴露于这些化学物质后，细胞存活率均呈剂量依赖性下降，尤其是Csb组肝细胞存活率低于Cs^a组。我们还观察到，皮肤美白乳膏的主要成分对苯二酚可剂量依赖性地增强细胞色素P450 2E1mRNA和蛋白的表达，并诱导细胞凋亡。

项目成果

Direct inhibition of arginase attenuated airway allergic reactions and inflammation in a Dermatophagoides farinae-induced NC/Nga mouse model

• DOI: 10.1152/ajplung.00216.2009
• 发表时间: 2010-07-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
• 影响因子: 4.9
• 作者: Takahashi, Noriko;Ogino, Keiki;Fujikura, Yoshihisa
• 通讯作者: Fujikura, Yoshihisa

新しい喘息予防法に関する研究

哮喘预防新方法研究

• 发表时间: 2011
• 作者: 張燃;村上育郎;高橋秀和;汪達紘; 荻野景規
• 通讯作者: 荻野景規

High Serum Arginase I Levels in Asthma: its Correlation with High-Sensitivity C-Reactive Protein

• DOI: 10.3109/02770903.2010.528496
• 发表时间: 2011-02-01
• 期刊: JOURNAL OF ASTHMA
• 影响因子: 1.9
• 作者: Ogino, Keiki;Obase, Yasushi;Oka, Mikio
• 通讯作者: Oka, Mikio

Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis.

• DOI: 10.1186/1471-2369-13-14
• 发表时间: 2012-03-25
• 期刊: BMC nephrology
• 影响因子: 2.3
• 作者: Takiue K;Sugiyama H;Inoue T;Morinaga H;Kikumoto Y;Kitagawa M;Kitamura S;Maeshima Y;Wang DH;Masuoka N;Ogino K;Makino H
• 通讯作者: Makino H

Evaluation of Pyrogallol-induced Cytotoxicity in Catalase-mutant Escherichia coli and Mutagenicity in Salmonella typhimurium. Bulletin of Environmental Contamination and Toxicology

邻苯三酚诱导的过氧化氢酶突变型大肠杆菌的细胞毒性和鼠伤寒沙门氏菌的致突变性评估。

• 发表时间: 2010
• 期刊: Bull. Environ. Contam. Toxicol
• 作者: Takemura Y;Wang DH;Sauriasari R;Horita M,Tsutsui K;Sano K;Masuoka N;Takigawa T;Takaki J;Ogino K
• 通讯作者: Ogino K