Development of novel functional oligonucleotide analogs for detection and regulation of a single base alteration in gene

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The congenital and acquired base mutations in genes are related to various diseases and to individual physical constitution. In this research, novel functional nucleic acid analogs were synthesized for development of a novel detection system for a single point mutation in gene and for suppression of cancer cell proliferation. Following results were obtained ;(1) Synthesis of novel functional nucleic acid analogs for gene diagnosis and gene regulation ;(2) Development of a protocol for homogeneous fluorescent assay of base alteration in RUNX1 and JAK2 genes ;(3) Selective inhibition of cancer cell proliferation by photodynamic antisense protocol ;(4) Development of live-cell RNA-imaging protocol. Through this research, we are convinced that the nucleic acids with sophisticated functions can reveal unknown functions of" RNA".