Atopic keratoconjunctivitis and natural helper cell

特应性角结膜炎和自然辅助细胞

• 批准号: 24659768
• 负责人: MURAKAMI Akira
• 金额: $ 2.33万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659768/
• 关键词: アトピー性角結膜炎; ナチュラルヘルパー細胞; IL-33; 自然免疫; パパイン; 春期カタル; アトピー; 結膜炎

We made innate immune system-dependent mouse conjunctivitis model using papain-absorbed contact lens. The lens was inserted into the conjunctival sac of B6 wild and B6 Rag2-kockout (KO) and IL-33 KO mice. In this mouse models, prominent eosinophilic infiltration was observed in the conjunctival tissue. The number of infiltrated eosinophils was significantly smaller in IL-33 KO mice compared to wild-type and Rag2-KO mice. These results suggested that IL-33 expression is essential for ocular surface allergic inflammatory process through the activation of innate immune systems. In addition, we detected natural helper (NH) cells in the lacrimal gland and conjunctival tissue of the innate immune system-dependent mouse conjunctivitis model. Moreover, we identified NH cell in the giant papillae tissue obtained from severe chronic allergic conjunctivitis patients. These results suggested that NH cell may participate the pathophysiological process of severe chronic allergic conjunctivitis.

采用木瓜蛋白酶吸附接触透镜制备小鼠先天免疫系统依赖性结膜炎模型。将透镜插入B6野生型和B6 Rag 2-kockout（KO）和IL-33 KO小鼠的结膜囊中。在该小鼠模型中，在结膜组织中观察到显著的嗜酸性粒细胞浸润。与野生型和Rag 2-KO小鼠相比，IL-33 KO小鼠中浸润的嗜酸性粒细胞数量显著较少。这些结果表明，IL-33的表达是必不可少的眼表过敏性炎症过程中通过激活先天免疫系统。此外，我们还检测了先天免疫系统依赖性结膜炎模型小鼠泪腺和结膜组织中的自然辅助（NH）细胞。此外，我们还鉴定了严重慢性过敏性结膜炎患者巨乳头组织中的NH细胞。提示NH细胞可能参与了重症慢性变应性结膜炎的病理生理过程。

项目成果

Interaction Between Conjunctival Epithelial Cells and Mast Cells Induces CCL2 Expression and Piecemeal Degranulation in Mast Cells

• DOI: 10.1167/iovs.12-10664
• 发表时间: 2013-04-01
• 期刊: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
• 影响因子: 4.4
• 作者: Iwamoto, Satoshi;Asada, Yosuke;Matsuda, Akira
• 通讯作者: Matsuda, Akira

Interleukin(IL)-33 and Thymic Stromal Lymphopoietin (TSLP), but not IL-25, induced Allergic Conjunctivitis in Mouse Models Papain-Induced Conjunctivitis

白介素 (IL)-33 和胸腺基质淋巴细胞生成素 (TSLP)（而非 IL-25）在小鼠模型中诱导过敏性结膜炎 木瓜蛋白酶诱导的结膜炎

• 发表时间: 2014
• 作者: Yosuke Asada;Akira Matsuda;Kanji Hori;Satoshi Iwamoto;Nobuyuki Ebihara;Akira Murakami;Susumu Nakae
• 通讯作者: Susumu Nakae

Detection of natural helper cells in innate immune system-dependent mouse conjunctivitis model

先天免疫系统依赖性小鼠结膜炎模型中自然辅助细胞的检测

• 发表时间: 2013
• 作者: Yosuke Asada;Akira Matsuda;Kanji Hori;Satoshi Iwamoto;Nobuyuki Ebihara;Akira Murakami;Susumu Nakae
• 通讯作者: Susumu Nakae

パパイン誘発結膜炎モデルマウスとナチュラルヘルパー細胞

木瓜蛋白酶诱导的结膜炎模型小鼠和自然辅助细胞

• 发表时间: 2013
• 作者: 浅田洋輔;松田 彰 他
• 通讯作者: 松田 彰 他

The role of interleukin-33 (IL-33) in mouse experimental allergic conjunctivitis

白介素33（IL-33）在小鼠实验性过敏性结膜炎中的作用

• 发表时间: 2013
• 作者: Akira Matsuda;Kanji Hori;Waka Ishida;Ken Fukuda;Nobuyuki Ebihara;Yousuke Asada;Susumu Nakae;Atsuki Fukushima;Akira Murakami. Keystone Symposia
• 通讯作者: Akira Murakami. Keystone Symposia