Developments of in situ real-time monitoring system by fluorescent-probe and regulation protocols of functional RNAs
Developments of in situ real-time monitoring system by fluorescent-probe and regulation protocols of functional RNAs
批准号:
19550164
负责人:
MURAKAMI Akira
金额:
$3.0万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2007
资助国家:
日本
项目状态:
已结题
起止时间:
2007 至 2008
中文摘要
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英文摘要
ヒトゲノムプロジェクトが明らかにした「RNA World」は、機能未知のRNAの存在を浮かび上がらせた。これらRNAの機能の解明は21世紀の生命科学の重要な課題である。本研究では独自開発した核酸分子素子を用い、機能性RNAを生細胞内で検出する手法開発ならびに特定のRNAの機能を選択的に制御する手法の開発を行った。その結果、生細胞内で特定のRNAのin situ検出に成功すると共に、1塩基変異を持つRNAの選択的機能制御に成功した。
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RNAプローブによる初期応答遺伝子の発現プロファイリング
使用 RNA 探针进行早期反应基因的表达谱分析
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
[村上章, 脇玲子, 坂本隆, 山吉麻子, 小堀哲生]
通讯作者:
小堀哲生
Naphthyridine-tethered Oligodeoxyribo-nucleotides : Dye/DNA conjugates for Homogeneous SNPs Assays
萘啶系链寡脱氧核糖核苷酸:用于均质 SNP 测定的染料/DNA 缀合物
DOI:
--
发表时间:
2008
期刊:
Chem. Lett. 37
影响因子:
--
作者:
[Kobori A., Mori T., Ubayashi M., MurakamiA.]
通讯作者:
MurakamiA.
Selective photo- cross-linked of 2'-O-psoralen-conjugated oligo- nucleotide with RNAs having point mutations
2-O-补骨脂素缀合寡核苷酸与具有点突变的 RNA 的选择性光交联
DOI:
--
发表时间:
2007
期刊:
Nucleosides, Nucleotides, & Nucleic Acids 26
影响因子:
--
作者:
[Higuchi M., Yamayoshi A., Yamaguchi T., Iwase T.,Yamaoka T., Murakami A.]
通讯作者:
Murakami A.
RNA 特異的検出プローブによるショウジョウバエ初期胚発現 mRNA の検出
使用 RNA 特异性检测探针检测早期果蝇胚胎中表达的 mRNA
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[上田貴子, 他]
通讯作者:
他
点突然変異陽性細胞における光架橋性アンチセンス核酸の細胞増殖抑制効果
光交联反义核酸对点突变阳性细胞的细胞增殖抑制作用
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[樋口麻衣子, 山吉麻子, 小堀哲生, 加藤聖子, 和氣徳夫, 村上章]
通讯作者:
村上章
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US Culture ( and Soft Power ) of the Cold War era and Turn-of-the-Century Cultural and Political Issues
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Development of RNA-diagnosis system for the analysisi of RNA and RNA-protein complexes
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Atopic keratoconjunctivitis and natural helper cell
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the Nationalism of the United States and Representations as Soft Power during the Cold War Era
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Asset management for the group of agricultural faclities based on the risk for soil erosion using the data assimilation
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Regulation of physiological functions with food factors via molecular chaperones
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IFN-γPAI-1 signals in ocular complications of atopic diseases
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Development of novel functional oligonucleotide analogs for detection and regulation of a single base alteration in gene
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Roles of adipocytokines in colorectal carcinogenesis and identification of preventive food factors
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System of Functional Diagnosis for Stock Management of Soil Structures Based on Integrated Measurement/Data Assimilation
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Pharmacological control of genetic deficient in corneal dystrophies
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Effects of lauric acid on the production of pro-inflammatory mediators and its risk assessment
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Measurement system of shear banding formation of granular materials based on the particle image velocimetry(PIV) with the image processing device and the meshless analysis system for bifurcation behavior of the specimen
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DESIGN OF FLUORESCENT RNA-PROBE FOR THE ANALYSIS OF THE HIGH-ORDER STRUCTURE OF RNA AND ITS APPLICATION TO THE GENE DESTRUCTION BY ANTISENSE OLIGONUCLEOTIDES
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Experimental and numerical approach for the behavior of granular medium within shear band
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Experimental and numerical approach for the behavior of granular medium within shear band
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R&D Studies of 3-dimentional particle tracking detector using CCD device for the particle physics
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海外基金