Analysis of neuronal impact properties for elucidationof pathogenesis of diffuse axonal injury(DAI)

本研究评估了PC12细胞的拉伸抗冲击能力。PC12细胞暴露在三种不同的拉伸中。然后，在显微镜下观察外伤性脑损伤的神经突长度、神经突数量、导致神经元断连而导致神经元死亡的肿胀数量，以及拉伸后5 ~ 24小时PC12细胞的细胞体数量。结果表明，暴露后神经突和细胞体减少，肿胀随时间增加。这些结果表明，拉伸后神经突肿胀和神经元死亡的产生与时间有关。

In this study, impact tolerance of PC12 cells for stretch was evaluated. PC12 cells were exposed to three different stretches. Then, the length of neurites, the number of neurites, the number of swellings which are the morphological hallmarks of pathology in traumatic brain injury and lead to the disconnection of neurons resulting in neuronal death, and the number of cell bodies in PC12 cells from 5 min to 24 hours after exposure to stretch were measured using microscope. As a result, the neurites and the cell bodies were decreased, and the swellings were increased time-dependently after the exposure. These results suggest that production of neurite swelling and neuronal death correlate with time course after the exposure of stretch.