Evaluation of the Degree of Maturation in the Regenerative Tissues by Analysis of Micro-Elastic Structure

我们已经开发了一种扫描触觉显微镜（SHM），便于观察组织切片样品表面弹性的分布。通过改进SHM，将猪动脉浸泡在生理盐水中，实现了长时间观察和广域表面弹性分布成像。因此，通过对犬主动脉不同节段细胞外基质水平局部弹性结构的可视化，揭示了空载条件下弹性结构的差异。SHM被证明是精确评估组织工程组织植入后物理成熟程度的有力工具。

We have been developed a scanning haptic microscope(SHM) for easy observation of the distribution of surface elasticity over a tissue slice sample. By improvement of SHM long time observation and wide area surface elasticity distribution imaging of the porcine arteries were enabled by soaking the sample in saline. Therefore, the differences in the elasticity under no load condition were revealed by visualization of local elastic structure at the extracellular matrix level in different segments of the canine aorta. SHM proved to be a powerful tool for precise evaluation of the degree of physical maturation after implantation of tissue-engineered tissues.