Function of the photo-induced triplet radical pair in the photo-sensor BLUF protein

光传感器 BLUF 蛋白中光诱导三重态自由基对的功能

• 批准号: 21370069
• 负责人: MINO Hiroyuki
• 金额: $ 12.31万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21370069/
• 关键词: 光生物; BLUF; 磁場効果; EPR; ESR; フラビン; センサータンパク質; ラジカル対; 時間分解EPR; 時間分EPR; BLUP

A photo-induced radical pair of FADH・and Y8・in BLUF protein SyPixD was studied by electron paramagnetic resonance(EPR) spectroscopy. The EPR signal has been characterized by a Pake doublet signal with complete S=1 spin state. The temperature dependence of the spin-lattice relaxation of the radical pair of flavin and tyrosine in SyPixD was investigated by pulsed electron paramagnetic resonance(EPR) spectroscopy. Based on the temperature dependence of spin. lattice relaxation time T-1, the exchange coupling of the radical pair was estimated as 2J=6 to 8 cm^<-1>, defined as-2JS-1* S-2.The radical pair was utilized as a probe to analyze the oligomer of SyPixD. The relative arrangement of PixD proteins in the complex was investigated by pulsed electron-electron double resonance(PELDOR) with the orientation selection. Based on the decameric structure in the crystal, the possible structure for the PELDOR results was evaluated.The transient EPR measurements have been performed to investigate the reaction pathway to produce radical-pair. The results show that the radical-pair was produced in the range of 200-263 K. The possible reaction mechanism was proposed.

用电子顺磁共振(EPR)研究了BLUF蛋白SyPixD中光诱导自由基对FADH·和Y8·。电子顺磁共振信号的特征是具有完整的S=1自旋态的帕克双重态。用脉冲电子顺磁共振(EPR)研究了SyPixD中黄素和酪氨酸自由基对的自旋-晶格驰豫与温度的关系。基于自旋对温度的依赖关系。晶格驰豫时间T~(-1)，自由基对的交换耦合为2J=6~8 cm~(-1)~(-1)，定义为-2J~(-1)*S~(-2)。用脉冲电子-电子双共振(PELDOR)结合取向选择研究了PixD蛋白在复合体中的相对排列。基于晶体中的十聚体结构，对PELDOR结果的可能结构进行了评估，并用瞬时EPR测量研究了生成自由基对的反应途径。结果表明，在200-263K的温度范围内产生了自由基对，并提出了可能的反应机理。

项目成果

ESEEM studies of the doublet signal with spin S = 1 in the blue light sensor BLUF protein SyPixD

蓝光传感器 BLUF 蛋白 SyPixD 中自旋 S = 1 的双峰信号的 ESEEM 研究

• 发表时间: 2010
• 作者: Kondo;T.;Masuda;S.;Mino;H.
• 通讯作者: H.

The position of the Mn^2+binding site at the first dark state for photoactivation in Photosystem II manganese cluster

Photosystem II 锰簇中用于光活化的第一暗状态下 Mn^2 结合位点的位置

• 发表时间: 2011
• 作者: Mizue Asada;Hiroyuki Mino
• 通讯作者: Hiroyuki Mino

Pulsed EPR analysis of the photo-induced triplet radical pair in the BLUF protein SyPixD : Determination of the protein-protein distance and orientation in the oligomeric protein

BLUF 蛋白 SyPixD 中光诱导三重态自由基对的脉冲 EPR 分析：测定寡聚蛋白中的蛋白-蛋白距离和方向

• DOI: 10.1007/s00723-011-0237-1
• 发表时间: 2011
• 期刊: Applied Magnetic Resonance
• 影响因子: 1
• 作者: Toru Kondo;Shinji Masuda and Hiroyuki Mino
• 通讯作者: Shinji Masuda and Hiroyuki Mino

The formation of an radical pair FADH.-Y8. in SyPixD investigated by pulsed and transient EPR

形成自由基对FADH.-Y8。

• 发表时间: 2010
• 作者: 三野広幸;近藤徹;筒井和彦;増田真二
• 通讯作者: 増田真二

法による青色光センサータンパク質SyPixD重合体のタンパク間相互作用の解析

蓝光传感器蛋白SyPixD聚合物的蛋白质-蛋白质相互作用分析方法

• 发表时间: 2009
• 作者: 近藤徹;筒井和彦;増田真二;三野広幸
• 通讯作者: 三野広幸