Analysis of GRWD1, a novel histone-binding protein involved in chromosomal replication initiation and segregation

GRWD1 的分析，一种参与染色体复制起始和分离的新型组蛋白结合蛋白

• 批准号: 21370084
• 负责人: FUJITA Masatoshi
• 金额: $ 8.99万
• 依托单位: Kyushu University (2010-2011)National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21370084/
• 关键词: GRWD1; Cdt1; 複製開始複合体; 細胞周期; ヒストンシャペロ; MCM; クロマチン; 次世代シークエンシング; 複製開始制御; ヒストンシャペロン; ライセンシング制御; 複製開始点

Precise regulation of DNA replication reaction is essential for integrity of genome information. Cdt1, together with ORC and CDC6, loads MCM replication helicase complex onto DNA replication origin. GRWD1 has been identified as a novel Cdt1-binding protein. In this research project, we have obtained following findings.(GRWD1 binds to Cdc6 and Cdt1, thereby accumulates onto replication origins.(2) GRWD1is a novel histone-binding protein and has a histone chaperon activity. Furthermore, GRWD1is actually involvedin regulation of chromatin openness(in general, it is believed thatopen chromatin structure promotes various transactions on chromatin).(3) GRWD1 silencing by siRNA inhibits MCM loading. Therefore, GRWD1may play a crucial role in regulation ofreplication.

DNA复制反应的精确调控对基因组信息的完整性至关重要。CDT1与ORC和CDC6一起将MCM复制解旋酶复合体装载到DNA复制起点上。GRWD1是一种新的CDT1结合蛋白。在本研究项目中，我们获得了以下发现：(GRWD1与CDC6和CDT1结合，从而聚集在复制起点上。(2)GRWD1是一种新的组蛋白结合蛋白，具有组蛋白伴侣活性。此外，GRWD1实际上参与了染色质开放的调节(一般认为，开放的染色质结构促进了染色质的各种交易)。(3)siRNA沉默GRWD1抑制了MCM的负载。因此，GRWD1可能在复制调控中发挥重要作用。

项目成果

The Cdt1-binding protein GRWD1 is a novel histone chaperone involved in replication licensing

Cdt1 结合蛋白 GRWD1 是一种参与复制许可的新型组蛋白伴侣

• 发表时间: 2011
• 作者: Nozomi Sugimoto;et al
• 通讯作者: et al

A chromatin remodeler SNF2H is involved in pre-RC formation and Cdt1-induced checkpoint activation

染色质重塑因子 SNF2H 参与前 RC 形成和 Cdt1 诱导的检查点激活

• 发表时间: 2009
• 作者: J. -G. Jee;Mizuno T;Kamada K;Tochio H;Chiba Y;Yanagi K;Yasuda G;Hiroaki H;Hanaoka F;Shirakawa M.;Takashi Yugawa;Fujita M.;Masatoshi Fujita;Kazumasa Yoshida;Nozomi Sugimoto
• 通讯作者: Nozomi Sugimoto

The Cdt1-binding protein GRWD1 is a novel histone chaperone involved in replication licensing.

Cdt1 结合蛋白 GRWD1 是一种参与复制许可的新型组蛋白伴侣。

• 发表时间: 2011
• 作者: Sugimoto;N.;Yasukouchi;S.;Watanabe;S.;Kiyono;T.;Kurumizaka;H. & Fujita;M.
• 通讯作者: M.

A novel function of ATM during the unperturbed S phase : Regulation of Cdt1 proteolysis and prevention of rereplication

ATM 在不受干扰的 S 期的新功能：调节 Cdt1 蛋白水解和预防再复制

• 发表时间: 2011
• 作者: Satoko Iwahori;et al
• 通讯作者: et al

The E1 Protein of Human Papillomavirus Type 16 Is Dispensable for Maintenance Replication of the Viral Genome

• DOI: 10.1128/jvi.06450-11
• 发表时间: 2012-03-01
• 期刊: JOURNAL OF VIROLOGY
• 影响因子: 5.4
• 作者: Egawa, Nagayasu;Nakahara, Tomomi;Kiyono, Tohru
• 通讯作者: Kiyono, Tohru