Analysis of mitochondrial function in mammalian oocytes using electrochemical microscopy

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Oxygen consumption is a ubiquitous parameter which can provide valuable informationon metabolic mechanisms, and oocyte and embryo quality. The aim of this study was 1) todevelop a device to measure cellular respiration of single oocytes and embryos based onscanning electrochemical microscopy (SECM) and 2) to assess the mitochondrial respiration function, such as oxygen consumption and gene expression of cytochrome coxidase (Cox) in single oocytes and embryos at different developmental stages. We succeeded in development of a modified SECM measuring system including Pt-microdiskelectrodes and respiration assay medium can monitor the oxygen consumption rate ofsingle oocytes and embryos. Using this system, we can measure respiration activity bysingle embryos and oocytes of bovine and mice at different developmental stages. Development of mitochondrial respiration activity, such as membrane potential of mitochondria and gene expression of Cox, corresponds to the increase of oxygen consumption rates during the development of embryos.