Horizontal transfer of pathogenicity islands of a virulence plasmid in Rhododoccus equi-Origin of the virulence-associated gene family-

马红球菌毒力质粒致病岛的水平转移毒力相关基因家族的起源-

• 批准号: 21380189
• 负责人: TAKAI Shinji
• 金额: $ 5.32万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380189/
• 关键词: Rhodococcus equi; 病原性プラスミド; 病原性遺伝子群; 水平伝播; 強毒株; 馬; 感染症; 起源; 進化; 適応; ロドコッカス・エクイ; 中等度毒力株; 潜在プラスミド; 病原性遣伝子群

Rhodococcus equi is a facultative, intracellular, Gram-positive coccobacillus that causes suppurative pneumonia and ulcerative enteritis in foals aged 1-3 months. This organism is present in soil and sporadically causes pulmonary abscesses and lymphadenitis in other domestic animals. The discovery of virulence-associated antigens and virulence plasmids has allowed the classification of R. equi. strains and molecular epidemiological studies of virulent R. equi. By the basis of restriction fragment patterns of representative strains of VapA-and VapB-positive R. equi digested by restriction enzymes, we found at least 15 distinct plasmid types in VapA-positive, and 33 types in VapB-positive R. equi. Nucleotide sequencing of the virulence plasmids of ATCC33701(the representative of VapA-positive) and A3(the representative of VapB-positive) revealed the presence of a pathogenicity island : it is characterised by a significantly lower G+C content than the rest of the plasmid, and is flankedby genes similar to transposon resolvases. The present study demonstraed that the determination and analysis of the nucleotide sequences of virulence plasmids provid the information on the diversity of virulence plasmids of R. equi.

马红球菌是一种兼性、细胞内、革兰氏阳性球杆菌，可引起1-3月龄马驹的化脓性肺炎和溃疡性肠炎。这种微生物存在于土壤中，偶尔会引起其他家畜的肺部肿胀和淋巴结炎。毒力相关抗原和毒力质粒的发现，使R.等。强毒R.等。根据VapA和VapB阳性R.经限制性内切酶酶切后，VapA阳性菌株中至少有15种不同的质粒类型，VapB阳性菌株中至少有33种不同的质粒类型。等。ATCC 33701（VapA阳性的代表）和A3（VapB阳性的代表）的毒力质粒的核苷酸测序显示存在一个致病岛：其特征是G+C含量显著低于质粒的其余部分，并且侧翼有类似于转座子解离酶的基因。本研究表明，毒力质粒核苷酸序列的测定和分析，为了解该菌毒力质粒的多样性提供了信息。等。

项目成果

Participation of CheR and CheB in the chemosensory response of Campylobacter jejuni

• DOI: 10.1099/mic.0.047399-0
• 发表时间: 2011-05-01
• 期刊: MICROBIOLOGY-SGM
• 影响因子: 2.8
• 作者: Kanungpean, Doungjit;Kakuda, Tsutomu;Takai, Shinji
• 通讯作者: Takai, Shinji

獣医微生物学

兽医微生物学

• 发表时间: 2012
• 作者: 見上彪;関崎勉;高井伸二;堀本泰介;望月雅美
• 通讯作者: 望月雅美

Genetic organization and preferential distribution of putative pilus gene clusters in Streptococcus suis

• DOI: 10.1016/j.vetmic.2009.02.013
• 发表时间: 2009-07-02
• 期刊: VETERINARY MICROBIOLOGY
• 影响因子: 3.3
• 作者: Takamatsu, Daisuke;Nishino, Hiroto;Sekizaki, Tsutomu
• 通讯作者: Sekizaki, Tsutomu

我が国の野生イノシシにおけるロドコッカス・エクイ中等度毒力株の分離

在日本野猪中分离出中等毒力的马红球菌菌株

• 发表时间: 2009
• 作者: 大野遼子;樋口ちひろ;岡野肇;須藤真登;鈴木和男;佐藤宏;前田健;佐々木由香子;角田勤;高井伸二
• 通讯作者: 高井伸二

Rhodococcus equi病原性プラスミド87kb II型の3種類の亜型の解析

马红球菌毒力质粒87kb II型三种亚型分析

• 发表时间: 2009
• 作者: 伊藤志野;川瀬将之;稲葉七巳;丹羽秀和;樋口徹;佐々木由香子;角田勤;高井伸二
• 通讯作者: 高井伸二