Application of iPS cells to the investigation of the pathogenesis of human retroviral infectious diseases and to the establishment of their prevention and therapy

我们开展这项研究是为了获得关于利用人类和猕猴iPS细胞预防病毒感染的新疗法和开发针对人类逆转录病毒传染病的治疗药物的基本信息。我们将HIV-1 gp160膜糖蛋白基因导入人iPS细胞，获得了该基因的高表达。HIV-1 gp160-iPS细胞接种小鼠可诱导显著的细胞免疫。因此，我们证明了人类iPS细胞作为新型艾滋病疫苗载体的可行性。我们将经过修饰的细胞因子引入人类iPS细胞，以抑制HIV的传播，并成功地建立了表达这两种基因的细胞系。我们从恒河猴真皮成纤维细胞中建立了iPS细胞，并成功地将其分化为血系干细胞。

We carried out this study in order to obtain basic information on the prevention of virus infection by novel therapies and on the development of therapeutic drugs against human retroviral infectious diseases utilizing human and macaque iPS cells. We introduced the HIV-1 gp160 membrane glycoprotein gene into human iPS cells, and obtained high expression of the gene. Remarkable cellular immunity was induced in HIV-1 gp160-iPS cells-inoculated mice. Thus we showed the feasibility of human iPS cells as a vector with superior performance for novel AIDS vaccines. We introduced into human iPS cells modified cellular factors engineered to inhibit the propagation of HIV, and succeeded to establish cell lines constitutively expressing either of the genes. We established iPS cells from rhesus monkey dermal fibroblasts, and succeeded to differentiate them to stem cells of blood lineage.