Cell culture system for genotype1b hepatitis C virus

丙型肝炎病毒感染会导致慢性肝病，是一个世界性的健康问题。尽管人们对病毒复制和致病机理的了解越来越多，但由于缺乏有效的病毒培养系统，对病毒生命周期的详细分析一直受到阻碍。在这项研究中，我们的目标是建立1b型丙型肝炎病毒培养体系，因为1b型感染是最常见的，并且对干扰素治疗耐药。我们分离了一个新的1b型丙型肝炎病毒克隆，并构建了病毒基因组的全长cDNA。通过引入适应性突变体，我们可以观察到病毒的复制和感染性病毒颗粒的形成和分泌。我们还利用基因1b和2a构建了嵌合病毒。利用该嵌合病毒构建物，我们分析了抗病毒敏感性图谱。结论：1b型丙型肝炎病毒培养体系对丙型肝炎病毒的抗病毒研究和耐药谱分析具有重要意义。

Hepatitis C virus(HCV) infection causes chronic liver diseases and is a worldwide health problem. Despite the increasing demand for the knowledge of viral replication and pathogenesis, detail analysis of viral life cycle has been hampered by the lack of efficient viral culture system. In this study, we aimed to establish genotype1b HCV culture system since genotype1b infection is most prevalent and resistant for interferon therapy. We isolate a new genotype1b HCV clone and constructed full-length cDNA of viral genome. By introduction of adaptive mutants, we could observe viral replication and infectious virus particle formation and secretion. We also constructed chimeric virus using genotype 1b and 2a. With this chimeric virus constructs, we analyzed the anti-viral sensitivity profiles. In conclusion, genotype1b HCV culture system is useful and important for anti-viral development and analysis of drug resistant profile of HCV.